Evidence map›Paper›PMID 29885581›Full record

ArticleBiosensors & bioelectronics2018

Application of bioconjugation chemistry on biosensor fabrication for detection of TAR-DNA binding protein 43.

Yifan Dai, Chunlai Wang, Liang-Yuan Chiu, Kevin Abbasi, Blanton S Tolbert, Geneviève Sauvé, Yun Yen, Chung-Chiun Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.2field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 45 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Yifan DaiDepartment of Chemical and Biomolecular Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA; Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
Chunlai WangDepartment of Chemistry, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
Liang-Yuan ChiuDepartment of Chemistry, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
Kevin AbbasiSwagelok Center for Surface Analysis of Materials (SCSAM), Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106, USA.
Blanton S TolbertDepartment of Chemistry, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
Geneviève SauvéDepartment of Chemistry, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
Yun YenTaipei Medical University, 250 Wuxing Street, Taipei City 110, Taiwan.
Chung-Chiun LiuDepartment of Chemical and Biomolecular Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA; Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA. Electronic address: cxl9@case.edu.
Case Western Reserve University · USTaipei Medical University · TW

Funding

STRUCTURAL BIOPHYSICS OF HIV ALTERNATIVE SPLICINGR01GM101979 · NIGMS · CASE WESTERN RESERVE UNIVERSITY · PI TOLBERT, BLANTON SMITH · 2012 to 2016
$1.6M
NIGMS NIH HHS R01 GM101979
6 · The paper itself

Abstract

A simple-prepare, single-use and cost-effective, in vitro biosensor for the detection of TAR DNA-binding protein 43 (TDP-43), a biomarker of neuro-degenerative disorders, was designed, manufactured and tested. This study reports the first biosensor application for the detection of TDP-43 using a novel biosensor fabrication methodology. Bioconjugation mechanism was applied by conjugating anti-TDP 43 with N-succinimidyl S-acetylthioacetate (SATA) producing a thiol-linked anti-TDP 43, which was used to directly link with gold electrode surface, minimizing the preparation steps for biosensor fabrication and simplifying the biosensor surface. The effectiveness of this bioconjugation mechanism was evaluated and confirmed by FqRRM12 protein, using nuclear magnetic resonance (NMR). The surface coverage of the electrode was analyzed by Time-of-Flight-Secondary Ion Mass Spectrometry (TOF-SIMS). Differential pulse voltammetry (DPV) was acted as the detection transduction mechanism with the use of [Fe(CN)

Indexed as

Biosensing TechniquesBlood Chemical AnalysisDNA-Binding ProteinsElectrodesGoldHumansNeurodegenerative DiseasesReproducibility of ResultsDNA-Binding ProteinsGoldTARDBP protein, humanBioconjugationDifferential pulse voltammetryElectrochemical detectionTAR DNA-binding protein 43 (TDP-43)

Identifiers

PMID29885581
PMCPMC6082407
OpenAlexW2806259911

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.