ArticleMolecular microbiology2018
Identifying a novel connection between the fungal plasma membrane and pH-sensing.
Article in Molecular microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Extracellular Yellow Pigments Produced byMicroorganisms · 2026Article
- Pathogenic Fungal Sensing and Responses to Stressful Host Environments.Annual review of microbiology · 2025Review
- Article
- Landscape of essential growth and fluconazole-resistance genes in the human fungal pathogen Cryptococcus neoformans.PLoS biology · 2025Article
- Improved Broad Spectrum Antifungal Drug Synergies with Cryptomycin, a Cdc50-Inspired Antifungal Peptide.ACS infectious diseases · 2024Article
- Malassezia responds to environmental pH signals through the conserved Rim/Pal pathway.bioRxiv : the preprint server for biology · 2024Article
- Cryptococcus neoformans adapts to the host environment through TOR-mediated remodeling of phospholipid asymmetry.Nature communications · 2023Article
- A fungal lytic polysaccharide monooxygenase is required for cell wall integrity, thermotolerance, and virulence of the fungal human pathogen Cryptococcus neoformans.PLoS pathogens · 2023Article
- Conserved and Divergent Features of pH Sensing in Major Fungal Pathogens.Current clinical microbiology reports · 2023Review
- A Peptide from Budding Yeast GAPDH Serves as a Promising Antifungal against Cryptococcus neoformans.Microbiology spectrum · 2022Article
- Comparative analysis of RNA enrichment methods for preparation of Cryptococcus neoformans RNA sequencing libraries.G3 (Bethesda, Md.) · 2021Article
- Article
- Human IgM Inhibits the Formation of Titan-Like Cells in Cryptococcus neoformans.Infection and immunity · 2020Article
- Article
- The cAMP/Protein Kinase a Pathway Regulates Virulence and Adaptation to Host Conditions inFrontiers in cellular and infection microbiology · 2019Review
- Role of lipid transporters in fungal physiology and pathogenicity.Computational and structural biotechnology journal · 2019Review
- TNF-α-induced Inhibition of Protein MyristoylationIn vivo (Athens, Greece)Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The mechanisms by which micro-organisms sense and internalize extracellular pH signals are not completely understood. One example of a known external pH-sensing process is the fungal-specific Rim/Pal signal transduction pathway. Fungi, such as the opportunistic pathogen Cryptococcus neoformans, use Rim signaling to sense and respond to changes in environmental pH. Mutations in this pathway result in strains that are attenuated for survival at alkaline pH, and often for survival within the host. Here, we used an insertional mutagenesis screen to identify novel genes required for C. neoformans growth at host pH. We discovered altered alkaline pH growth in several strains with specific defects in plasma membrane composition and maintenance of phospholipid assembly. Among these, loss of function of the Cdc50 lipid flippase regulatory subunit affected the temporal dynamics of Rim pathway activation. We defined distinct and overlapping cellular processes regulated by Rim101 and Cdc50 through analysis of the transcriptome in these mutant strains. We further explored how pH-induced membrane changes affect membrane-bound pH-sensing proteins, specifically the C-terminal domain of the Rra1 protein, an upstream Rim pathway activator and pH sensor. These results suggest both broadly applicable and phylum-specific molecular interactions that drive microbial environmental sensing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.