ArticleScientific reports2018
Impact of physical confinement on nuclei geometry and cell division dynamics in 3D spheroids.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- A customizable, low-cost 3D-printed device for live cell confinement imaging.Lab on a chip · 2026Article
- Beyond monolayers: a comparative analysis of 2D cell cultures and 3DFrontiers in bioengineering and biotechnology · 2026Review
- Mitochondria-derived nuclear ATP surge protects against confinement-induced proliferation defects.Nature communications · 2025Article
- Improving 3D deep learning segmentation with biophysically motivated cell synthesis.Communications biology · 2025Article
- Hypoxic postconditioning modulates neuroprotective glial reactivity in a 3D cortical ischemic-hypoxic injury model.Scientific reports · 2024Article
- Mechanical confinement prevents ectopic platelet release.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Depth-enhanced high-throughput microscopy by compact PSF engineering.Nature communications · 2024Article
- Article
- The role of cell-matrix adhesion and cell migration in breast tumor growth and progression.Frontiers in cell and developmental biology · 2024Article
- Applications and Advances of Multicellular Tumor Spheroids: Challenges in Their Development and Analysis.International journal of molecular sciences · 2023Review
- Article
- Cell3: a new vision for study of the endomembrane system in mammalian cells.Bioscience reports · 2021Review
- Article
- Single cell organization and cell cycle characterization of DNA stained multicellular tumor spheroids.Scientific reports · 2021Article
- Understanding the influence of substrate when growing tumorspheres.BMC cancer · 2021Article
- Adhesion-growth factor crosstalk regulates AURKB activation and ERK signalling in re-adherent fibroblasts.Journal of biosciences · 2021Article
- Perspective: Extending the Utility of Three-Dimensional Organoids by Tissue Clearing Technologies.Frontiers in cell and developmental biology · 2021Article
- Influence ofInternational journal of molecular sciences · 2020Article
- Slicing Spheroids in Microfluidic Devices for Morphological and Immunohistochemical Analysis.Micromachines · 2020Article
- Routine Optical Clearing of 3D-Cell Cultures: Simplicity Forward.Frontiers in molecular biosciences · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multicellular tumour spheroids are used as a culture model to reproduce the 3D architecture, proliferation gradient and cell interactions of a tumour micro-domain. However, their 3D characterization at the cell scale remains challenging due to size and cell density issues. In this study, we developed a methodology based on 3D light sheet fluorescence microscopy (LSFM) image analysis and convex hull calculation that allows characterizing the 3D shape and orientation of cell nuclei relative to the spheroid surface. By using this technique and optically cleared spheroids, we found that in freely growing spheroids, nuclei display an elongated shape and are preferentially oriented parallel to the spheroid surface. This geometry is lost when spheroids are grown in conditions of physical confinement. Live 3D LSFM analysis of cell division revealed that confined growth also altered the preferential cell division axis orientation parallel to the spheroid surface and induced prometaphase delay. These results provide key information and parameters that help understanding the impact of physical confinement on cell proliferation within tumour micro-domains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.