ArticleScientific reports2018
Mutations in blind cavefish target the light-regulated circadian clock gene, period 2.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Convergent latitudinal erosion of circadian systems in a rapidly diversifying order of fishes.PLoS genetics · 2026Article
- Conservation of dark CPD photolyase function in blind cavefish.Nature communications · 2025Article
- A repeatedly evolved mutation in Cryptochrome-1 of subterranean animals alters behavioral and molecular circadian rhythms.iScience · 2025Article
- Sporadic feeding regulates robust food entrainable circadian clocks in blind cavefish.iScience · 2024Article
- Ontogeny and social context regulate the circadian activity patterns of Lake Malawi cichlids.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2024Article
- Blind fish have cells that see light.Proceedings. Biological sciences · 2023Article
- Alternative splicing as a source of phenotypic diversity.Nature reviews. Genetics · 2022Review
- CaveCrawler: an interactive analysis suite for cavefish bioinformatics.G3 (Bethesda, Md.) · 2022Article
- Enhanced lipogenesis through Pparγ helps cavefish adapt to food scarcity.Current biology : CB · 2022Article
- Article
- Repeated evolution of circadian clock dysregulation in cavefish populations.PLoS genetics · 2021Article
- Finding Nemo's clock reveals switch from nocturnal to diurnal activity.Scientific reports · 2021Article
- Adaptation and convergence in circadian-related genes in Iberian freshwater fish.BMC ecology and evolution · 2021Article
- Contrasting Gene Decay in Subterranean Vertebrates: Insights from Cavefishes and Fossorial Mammals.Molecular biology and evolution · 2021Article
- Evolution Shapes the Gene Expression Response to Oxidative Stress.International journal of molecular sciences · 2019Review
- Circadian Clocks in Fish-What Have We Learned so far?Biology · 2019Review
- Differential circadian and light-driven rhythmicity of clock gene expression and behaviour in the turbot, Scophthalmus maximus.PloS one · 2019Article
- Time-Lag in Feeding Schedule Acts as a Stressor That Alters Circadian Oscillators in Goldfish.Frontiers in physiology · 2018Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light represents the principal signal driving circadian clock entrainment. However, how light influences the evolution of the clock remains poorly understood. The cavefish Phreatichthys andruzzii represents a fascinating model to explore how evolution under extreme aphotic conditions shapes the circadian clock, since in this species the clock is unresponsive to light. We have previously demonstrated that loss-of-function mutations targeting non-visual opsins contribute in part to this blind clock phenotype. Here, we have compared orthologs of two core clock genes that play a key role in photic entrainment, cry1a and per2, in both zebrafish and P. andruzzii. We encountered aberrantly spliced variants for the P. andruzzii per2 transcript. The most abundant transcript encodes a truncated protein lacking the C-terminal Cry binding domain and incorporating an intronic, transposon-derived coding sequence. We demonstrate that the transposon insertion leads to a predominantly cytoplasmic localization of the cavefish Per2 protein in contrast to the zebrafish ortholog which is distributed in both the nucleus and cytoplasm. Thus, it seems that during evolution in complete darkness, the photic entrainment pathway of the circadian clock has been subject to mutation at multiple levels, extending from opsin photoreceptors to nuclear effectors.
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