ArticleScientific reports2018
Far rapid synthesis of giant DNA in the Bacillus subtilis genome by a conjugation transfer system.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Synthetic rewriting technologies in mammalian cells.Nature communications · 2026Review
- The design and engineering of synthetic genomes.Nature reviews. Genetics · 2025Review
- XanthoMoClo─A Robust Modular Cloning Genetic Toolkit for the GeneraACS synthetic biology · 2025Article
- Synthetic biology in plants.Plant biotechnology (Tokyo, Japan) · 2024Article
- Integrated conjugal plasmid pLS20 in the Bacillus subtilis genome produced 850-kbp circular subgenomes transmissible to another B. subtilis.Genes to cells : devoted to molecular & cellular mechanisms · 2024Article
- Addressable and adaptable intercellular communication via DNA messaging.Nature communications · 2023Article
- Cross-species microbial genome transfer: a Review.Frontiers in bioengineering and biotechnology · 2023Review
- A CRISPR-based chromosomal-separation technique for Escherichia coli.Microbial cell factories · 2022Article
- Conjugation-Based Genome Engineering inACS synthetic biology · 2022Article
- Synthetic Genomics From a Yeast Perspective.Frontiers in bioengineering and biotechnology · 2022Review
- SEVA 3.1: enabling interoperability of DNA assembly among the SEVA, BioBricks and Type IIS restriction enzyme standards.Microbial biotechnology · 2020Article
- Factors That Affect the Enlargement of Bacterial Protoplasts and Spheroplasts.International journal of molecular sciences · 2020Review
- Integration of large heterologous DNA fragments into the genome of Thermococcus kodakarensis.Extremophiles : life under extreme conditions · 2020Article
- Trans-Kingdom Conjugation within Solid Media fromInternational journal of molecular sciences · 2019Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus subtilis offers a platform for giant DNA synthesis, which is mediated by the connection of overlapping DNA segments called domino DNA, in the cloning locus of the host. The domino method was successfully used to produce DNA fragments as large as 3500 kbp. However, domino DNA is limited to <100 kbp because of size restrictions regarding the transformation (TF) of B. subtilis competent cells. A novel conjugal transfer (CT) method was designed to eliminate the TF size limit. The CT method enables rapid and efficient domino reactions in addition to the transfer of giant DNA molecules of up to 875 kbp to another B. subtilis genome within 4 hours. The combined use of the TF and CT should enable significantly rapid giant DNA production.
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