Evidence map›Paper›PMID 29884615›Full record

ArticleG3 (Bethesda, Md.)2018

A Dual sgRNA Approach for Functional Genomics in

Laurens Pauwels, Rebecca De Clercq, Jonas Goossens, Sabrina Iñigo, Clara Williams, Mily Ron, Anne Britt, Alain Goossens

Erratum issuedAbstract read
In one paragraph

Article in G3 (Bethesda, Md.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

  1. Article
  2. Efficient Knocking Out of the Organophosphorus Insecticides Degradation GeneInternational journal of molecular sciences · 2023
    Article
  3. The Non-JAZ TIFY Protein TIFY8 ofInternational journal of molecular sciences · 2023
    Article
  4. The ArabidopsisPlant direct · 2023
    Article
  5. Review
  6. ThePlants (Basel, Switzerland) · 2022
    Article
  7. Article
  8. Article
  9. Review
  10. Article
  11. Article
  12. Review
  13. CRISPR/Cas9-Mediated Targeted Mutagenesis ofFrontiers in plant science · 2021
    Article
  14. First Report of CRISPR/Cas9 Gene Editing inFrontiers in plant science · 2021
    Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Laurens PauwelsGhent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium lapau@psb.ugent.be.ORCID 0000-0002-0221-9052
Rebecca De ClercqGhent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.
Jonas GoossensGhent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.ORCID 0000-0002-7940-2699
Sabrina IñigoGhent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.ORCID 0000-0001-5820-7109
Clara WilliamsGhent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.ORCID 0000-0003-1292-6313
Mily RonUC Davis, Department of Plant Biology, Davis, CA 95616, US.ORCID 0000-0003-1682-7275
Anne BrittUC Davis, Department of Plant Biology, Davis, CA 95616, US.
Alain GoossensGhent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium.ORCID 0000-0002-1599-551X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reverse genetics uses loss-of-function alleles to interrogate gene function. The advent of CRISPR/Cas9-based gene editing now allows the generation of knock-out alleles for any gene and entire gene families. Even in the model plant

Indexed as

MutagenesisArabidopsisArabidopsis ProteinsCRISPR-Cas SystemsDNA-Directed DNA PolymeraseDNA End-Joining RepairDNA Polymerase thetaGene EditingGenomicsRNA, Guide, CRISPR-Cas SystemsArabidopsis ProteinsDNA-Directed DNA PolymeraseDNA Polymerase thetaRNA, Guide, CRISPR-Cas Systemsalt-EJArabidopsis thalianaCRISPR/Cas9genome editinggenome engineeringpolymerase thetaRNA-guided nuclease

Identifiers

PMID29884615
PMCPMC6071589

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.