ArticleBMC veterinary research2018
Exploration of serum- and cell culture-derived exosomes from dogs.
Article in BMC veterinary research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
22 citing papers in PubMed.
- Extracellular Vesicles as Emerging Tools in Canine Mammary Tumors: Potential Applications and Perspectives.Animals : an open access journal from MDPI · 2026Review
- Proteomic and metabolomic profiles of plasma-derived Extracellular Vesicles differentiate melanoma patients from healthy controls.Translational oncology · 2024Article
- Antibacterial Effects of Essential Oils onPharmaceuticals (Basel, Switzerland) · 2024Article
- Serum Extracellular Vesicles Cargo Approach in Bitches with Mammary Tumors.Current issues in molecular biology · 2024Article
- Exploring Canine Mammary Cancer through Liquid Biopsy: Proteomic Profiling of Small Extracellular Vesicles.Cancers · 2024Article
- Canine Mesenchymal Stromal Cell Exosomes: State-of-the-Art Characterization, Functional Analysis and Applications in Various Diseases.Veterinary sciences · 2024Review
- Isolation and usage of exosomes in central nervous system diseases.CNS neuroscience & therapeutics · 2024Review
- Analysis of Plasma-Derived Exosomal MicroRNAs as Potential Biomarkers for Canine Idiopathic Epilepsy.Animals : an open access journal from MDPI · 2024Article
- Extracellular Vesicles in Veterinary Medicine.Animals : an open access journal from MDPI · 2022Review
- Evaluation of circulating extracellular vesicles and miRNA in neutered and obese female dogs.Scientific reports · 2022Article
- Systematic analysis of different degrees of haemolysis on miRNA levels in serum and serum-derived extracellular vesicles from dogs.BMC veterinary research · 2022Article
- Isolation and identification of exosomes from feline plasma, urine and adipose-derived mesenchymal stem cells.BMC veterinary research · 2021Article
- Extracellular Vesicles: Novel Opportunities to Understand and Detect Neoplastic Diseases.Veterinary pathology · 2021Review
- Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids.Scientific reports · 2021Article
- Elucidating Methods for Isolation and Quantification of Exosomes: A Review.Molecular biotechnology · 2021Review
- Circulating Melanoma-Derived Extracellular Vesicles: Impact on Melanoma Diagnosis, Progression Monitoring, and Treatment Response.Pharmaceuticals (Basel, Switzerland) · 2020Review
- Review
- Canine colostrum exosomes: characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity.BMC veterinary research · 2020Article
- Altered Basal Autophagy Affects Extracellular Vesicle Release in Cells of Lagotto Romagnolo Dogs With a VariantVeterinary pathology · 2020Article
- Deregulation of miR-27a may contribute to canine fibroblast activation after coculture with a mast cell tumour cell line.FEBS open bio · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExosomes are defined as extracellular membrane vesicles, 30-150 nm in diameter, derived from all types of cells. They originate via endocytosis and then they are released through exocytosis to the extracellular space, being found in various biological fluids as well as in cell culture medium. In the last few years, exosomes have gained considerable scientific interest due to their potential use as biomarkers, especially in the field of cancer research. This report describes a method to isolate, quantify and identify serum- and cell culture-derived exosomes from dog samples, using small volumes (100 μL and 1 mL, respectively).
resultsQuantification and sizing of exosomes contained in serum and cell culture samples were assessed by utilizing nanoparticle tracking analysis, transmission electron microscopy and immunoelectron microscopy. Detected particles showed the normal size (30-150 nm) and morphology described for exosomes, as well as presence of the transmembrane protein CD63 known as exosomal marker.
conclusionsBased on a validated rapid isolation procedure of nanoparticles from small volumes of different types of dog samples, a characterization and exploration of intact exosomes, as well as facilitation for their analysis in downstream applications was introduced.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.