ArticlePloS one2018
Absorbance summation: A novel approach for analyzing high-throughput ELISA data in the absence of a standard.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
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- Vaccination-Route-Dependent Adjuvanticity of Antigen-Carrying Nanoparticles for Enhanced Vaccine Efficacy.Vaccines · 2024Article
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- Dendritic Cell-Mediated Cross-Priming by a Bispecific Neutralizing Antibody Boosts Cytotoxic T Cell Responses and Protects Mice against SARS-CoV-2.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Emulsion and liposome-based adjuvanted R21 vaccine formulations mediate protection against malaria through distinct immune mechanisms.Cell reports. Medicine · 2023Article
- A chikungunya virus-like particle vaccine induces broadly neutralizing and protective antibodies against alphaviruses in humans.Science translational medicine · 2023Article
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- SARS-CoV-2 spike HexaPro formulated in aluminium hydroxide and administered in an accelerated vaccination schedule partially protects Syrian Hamsters against viral challenge despite low neutralizing antibody responses.Frontiers in immunology · 2023Article
- From a recombinant key antigen to an accurate, affordable serological test: Lessons learnt from COVID-19 for future pandemics.Biochemical engineering journal · 2022Article
- Immunogenicity of SARS-CoV-2 Trimeric Spike Protein Associated to Poly(I:C) Plus Alum.Frontiers in immunology · 2022Article
- Development and Characterization of Monoclonal Antibodies for the Detection of Fish Protein.Foods (Basel, Switzerland) · 2021Article
- SARS-CoV-2 Serologic Assays in Control and Unknown Populations Demonstrate the Necessity of Virus Neutralization Testing.The Journal of infectious diseases · 2021Observational
- A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies.Nature communications · 2021Article
- Distinct antibody responses to SARS-CoV-2 in children and adults across the COVID-19 clinical spectrum.Nature immunology · 2021Article
- CXCR5Frontiers in immunology · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
We have developed a very simple method, termed absorbance summation (AS), for comparing protein concentrations between samples in ELISA assays without a standard. This method sums the observed absorbance values from all dilutions to obtain one data point for each sample to be used for comparison. AS is less computationally intensive than fitting sigmoidal curves, and it avoids the difficulty of parameter estimation for samples with absorbance values lying primarily at the lower tail of the curve. Our simulation studies showed that it performs much better than the sigmoidal curve fitting method and the conventional endpoint titer method. The power of this simple method is as high as the formal curve fitting followed by the estimation of area under the curve (AUC).
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