ArticleJournal of the American Chemical Society2018
Single Synaptic Observation of Cholinergic Neurotransmission on Living Neurons: Concentration and Dynamics.
Article in Journal of the American Chemical Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Who cites it
30 citing papers in PubMed.
- GluD1 is localized at cholinergic synapses and is an acetylcholine receptor.Molecular psychiatry · 2026Article
- Advances in scanning probe microscopy for biological systems.Biophysics reports · 2026Article
- Observation of Acetylcholine Secretion on Living Glioblastoma Cells.ACS measurement science au · 2026Article
- Review
- Recent advances of in situ and in vivo electrochemical analysis of brain chemistry at micro- and nanoscale.QRB discovery · 2026Review
- Selective Detection of Acetylcholine against Choline and In Vivo Measurement in the Mouse Brain Using the Micropipet-Supported Liquid/Liquid Interface Electrode.ACS measurement science au · 2025Article
- In vivo dynamic tracking of cerebral chloride regulation using molecularly tailored liquid/liquid interfacial ultramicro iontronics.Science advances · 2024Article
- Nanoscale Quantitative Imaging of Single Nuclear Pore Complexes by Scanning Electrochemical Microscopy.Analytical chemistry · 2024Article
- Transient theory for scanning electrochemical microscopy of biological membrane transport: uncovering membrane-permeant interactions.The Analyst · 2024Article
- Nanoelectrochemistry at liquid/liquid interfaces for analytical, biological, and material applications.Chemical communications (Cambridge, England) · 2023Review
- Two-photon fluorescence imaging and specifically biosensing of norepinephrine on a 100-ms timescale.Nature communications · 2023Article
- Micro- and nano-devices for electrochemical sensing.Mikrochimica acta · 2022Review
- Carbon microelectrodes with customized shapes for neurotransmitter detection: A review.Analytica chimica acta · 2022Review
- Chemical Imaging and Analysis of Single Nerve Cells by Secondary Ion Mass Spectrometry Imaging and Cellular Electrochemistry.Frontiers in synaptic neuroscience · 2022Review
- Imaging in vivo acetylcholine release in the peripheral nervous system with a fluorescent nanosensor.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Chemical Analysis of Single Cells and Organelles.Analytical chemistry · 2021Review
- Nanoelectrochemical quantification of single-cell metabolism.Analytical and bioanalytical chemistry · 2021Review
- 3D-Printed Carbon Nanoelectrodes for In Vivo Neurotransmitter Sensing.Nano letters · 2020Article
- Nanoelectrochemistry in the study of single-cell signaling.Analytical and bioanalytical chemistry · 2020Review
- The double life of conductive nanopipette: a nanopore and an electrochemical nanosensor.Chemical science · 2020Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Acetylcholine, the first neurotransmitter identified more than a century ago, plays critical roles in human activities and health; however, its synaptic concentration dynamics have remained unknown. Here, we demonstrate the in situ simultaneous measurements of synaptic cholinergic transmitter concentration and release dynamics. We used nanoscale electroanalytical methods: nanoITIES electrode of 15 nm in radius and nanoresolved scanning electrochemical microscopy (SECM). Time-resolved in situ measurements unveiled information on synaptic acetylcholine concentration and release dynamics of living Aplysia neurons. The measuring technique enabled the quantitative sensing of acetylcholine with negligible interference of other ionic and redox-active species. We measured cholinergic transmitter concentrations very close to the synapse, with values as high as 2.4 mM. We observed diverse synaptic transmitter concentration dynamics consisting of singlet, doublet and multiplet events with a signal-to-noise ratio of 6 to 130. The unprecedented details about synaptic neurotransmission unveiled are instrumental for understanding brain communication and diseases in a way distinctive from extra-synaptic studies.
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Registered trials
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