Evidence map›Paper›PMID 29882149›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Isolation and Identification of Innate Lymphoid Cells (ILCs) for Immunotoxicity Testing.

Elia D Tait Wojno, Celine A Beamer

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Cytometry profiling ofFrontiers in immunology · 2023
    Article
  6. Oral exposure toFrontiers in immunology · 2023
    Article
  7. ILC3: a case of conflicted identity.Frontiers in immunology · 2023
    Review
  8. Review
  9. Insights into the tumor microenvironment of B cell lymphoma.Journal of experimental & clinical cancer research : CR · 2022
    Review
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Elia D Tait WojnoBaker Institute for Animal Health and Department of Microbiology and Immunology, Cornell University College of Veterinary Medicine, Ithaca, NY, USA.
Celine A BeamerDepartment of Biomedical and Pharmaceutical Sciences, Center for Biomolecular Structure and Dynamics, The University of Montana, Missoula, MT, USA. celine.beamer@umontana.edu.

Funding

Surveillance genome sequencing to detect SARS-CoV-2 virus variants in MontanaP20GM103546 · NIGMS · UNIVERSITY OF MONTANA · PI BOWLER, BRUCE E · 2012 to 2021
$19.2M
Role of the prostaglandin D2 receptor CRTH2 in helminth-induced type 2 inflammation in the intestine.R01AI130379 · NIAID · UNIVERSITY OF WASHINGTON · PI Elia D Tait Wojno · 2017 to 2026
$3.5M
Understanding how activation of the aryl hydrocarbon receptor (AhR) regulates IL-22 production in type 3 innate lymphoid cells (ILC3s)R15ES027648 · NIEHS · UNIVERSITY OF MONTANA · PI BEAMER, CELINE A · 2017 to 2018
$517k
Prostaglandin regulation of type 2 inflammationK22AI116729 · NIAID · CORNELL UNIVERSITY · PI TAIT WOJNO, ELIA D · 2016 to 2017
$267k
NIAID NIH HHS K22 AI116729NIAID NIH HHS R01 AI130379NIEHS NIH HHS R15 ES027648NIGMS NIH HHS P20 GM103546
6 · The paper itself

Abstract

Innate lymphoid cells (ILCs) comprise a family of innate immune cells that orchestrate mucosal immune responses: initiating, sustaining, and even curbing immune responses. ILCs are relatively rare (≤1% of lymphocytes in mucosal tissues), lack classical cell-surface markers, and can be divided into three subsets (type 1-3 ILCs) based on differences in cytokine production, phenotype, and developmental pathway. Because ILCs can only be identified by combinations of cell-surface markers and cytokine production, multicolor flow cytometry is the most reliable method to purify, characterize, and assess the functionality of ILCs. Here, we describe the methods for cell preparation, flow cytometric analysis, and purification of murine ILCs from the lung.

Indexed as

Immunity, InnateAdoptive TransferAnimalsCell ProliferationCell SeparationFlow CytometryLungLymphocytesMagneticsMice, Inbred C57BLStaining and LabelingToxicity TestsFlow cytometryILC2ILC3Innate lymphoid cellsMouseRespiratory tract

Identifiers

PMID29882149
PMCPMC6025753

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.