Evidence map›Paper›PMID 29881953›Full record

ArticleProbiotics and antimicrobial proteins2019

Safety Evaluation and Colonisation Abilities of Four Lactic Acid Bacteria as Future Probiotics.

Ziyanda C Dlamini, Rashwahla L S Langa, Olayinka A Aiyegoro, Anthony I Okoh

Abstract read
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In one paragraph

Article in Probiotics and antimicrobial proteins, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed.

  1. Exploring the Adhesion Potential ofFoods (Basel, Switzerland) · 2026
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  6. Screening of lactic acid bacteria from the milk of Sahiwal cows and characterization of their probiotic potential for preventing bovine mastitis.International microbiology : the official journal of the Spanish Society for Microbiology · 2025
    Article
  7. Article
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  9. In Vitro Analysis of Probiotic Properties Related to the Adaptation ofInternational journal of molecular sciences · 2025
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  11. Camel milkFrontiers in nutrition · 2025
    Article
  12. Article
  13. Article
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  15. Article
  16. Isolation, characterization of Weissella confusa and Lactococcus lactis from different milk sources and determination of probiotic features.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024
    Article
  17. Antioxidant Effects and Probiotic Properties ofFoods (Basel, Switzerland) · 2023
    Article
  18. Article
  19. Probiotic Characteristics and Safety Assessment ofJournal of microbiology and biotechnology · 2023
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ziyanda C DlaminiAgricultural Research Council - Animal Production Institute, Gastrointestinal Microbiology and Biotechnology Division, Irene, 0062, South Africa.
Rashwahla L S LangaAgricultural Research Council - Animal Production Institute, Gastrointestinal Microbiology and Biotechnology Division, Irene, 0062, South Africa.
Olayinka A AiyegoroAgricultural Research Council - Animal Production Institute, Gastrointestinal Microbiology and Biotechnology Division, Irene, 0062, South Africa. AiyegoroO@arc.agric.za.ORCID 0000-0001-6664-6574
Anthony I OkohSAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice, 5700, South Africa.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The study evaluated the safety and colonisation properties of four lactic acid bacteria (LAB), by determining their cell hydrophobicity and aggregation abilities. In addition, the presence of virulence and resistance genes was assayed in these probiotic candidates. Lactobacillus reuteri ZJ625, Lactobacillus reuteri VB4, Lactobacillus salivarius ZJ614 and Streptococcus salivarius NBRC13956 were tested for cell surface hydrophobicity abilities against xylene, chloroform and ethyl acetate. The isolates were also tested for auto-aggregation and co-aggregation abilities; the optical densities of cell growth were measured after 1, 2, 3 and 4 h of experimental set-up. DNA was extracted from all the four isolates and amplified using PCR with specific primers to detect virulence genes of adhesion collagen protein (ace) and aggregation substances (agg and asa); also, resistance genes of Vancomycin vanA, Vancomycin vanC1 and Vancomycin vanC2/3 were assayed in the four isolates. The isolates showed high hydrophobicity to all solvents: xylene (78-84%), chloroform (68-75%) and ethyl acetate (52-60%). High auto- and co-aggregations ranging from 60 to 70% and from 45 to 56% respectively were observed in the isolates after 4 h of incubation at 37 °C. Some of the tested isolates showed the presence of virulence and resistance genes; however, this does not indicate that these genes are unsafe because their transmission and expression abilities are unknown. Therefore, in this study, the isolates studied are considered safe for use as future probiotics, as revealed from results presented, which generally represents the scanned safety evaluations of the isolates as promising probiotics.

Indexed as

Hydrophobic and Hydrophilic InteractionsLactobacillalesProbioticsVirulenceAuto-aggregationCo-aggregationHydrophobicityProbioticsResistant genesVirulent genes

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.