ArticlePlant methods2018
Selection and evaluation of reference genes for qRT-PCR analysis in
Article in Plant methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 60 citations in OpenAlex.
- Identification of Reference Genes and Their Application in Analysis of Ginsenoside Biosynthesis Genes in American Ginseng Under Abiotic Stresses and Hormone Treatments.Plants (Basel, Switzerland) · 2026Article
- Reference Gene Stability inInternational journal of molecular sciences · 2026Article
- Selection and validation of suitable reference genes for qPCR expression analysis in youngFrontiers in plant science · 2026Article
- Identification and validation of reference genes for qPCR of Pseudomonas aeruginosa L10 under varying n-hexadecane concentrations.BMC microbiology · 2025Article
- Selection and Validation of Reference Genes inPlants (Basel, Switzerland) · 2024Article
- Selection and Validation of qRT-PCR Internal Reference Genes to Study Flower Color Formation inInternational journal of molecular sciences · 2024Article
- Screening and validation of optimal miRNA reference genes in different developing stages and tissues of Lilium henryi Baker.Scientific reports · 2024Article
- Article
- Grapevine scion gene expression is driven by rootstock and environment interaction.BMC plant biology · 2023Article
- Identification and Validation of Reference Genes for Expression Analysis Using qRT-PCR inInsects · 2022Article
- Article
- Selection and Validation of Reference Genes for Quantitative Real-Time PCR Analysis of Development and Tissue-Dependent Flower Color Formation inInternational journal of molecular sciences · 2022Article
- Multi-dimensional leaf phenotypes reflect root system genotype in grafted grapevine over the growing season.GigaScience · 2021Article
- Public transcriptome database-based selection and validation of reliable reference genes for breast cancer research.Biomedical engineering online · 2021Article
- The Euscaphis japonica genome and the evolution of malvids.The Plant journal : for cell and molecular biology · 2021Article
- Identification and validation of reference genes for qRT-PCR based studies in horse gram (Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Identification and selection of reference genes for gene expression analysis by quantitative real-time PCR in Suaeda glauca's response to salinity.Scientific reports · 2021Article
- Genome-Wide Identification and Co-Expression Analysis ofFrontiers in genetics · 2021Article
- Validation of Reference Genes for Quantitative Real-Time PCR Normalization inFrontiers in genetics · 2021Article
- Transcriptome-based selection and validation of optimal house-keeping genes for skin research in goats (Capra hircus).BMC genomics · 2020Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundQuantitative real-time reverse transcription-polymerase chain reaction has been widely used in gene expression analysis, however, to have reliable and accurate results, reference genes are necessary to normalize gene expression under different experimental conditions. Several reliable reference genes have been reported in plants of Traditional Chinese Medicine, but none have been identified for
resultsIn this study, 12 candidate reference genes, including 3 common housekeeping genes and 9 novel genes based on
conclusionResults of this study revealed that suitable reference genes should be selected for different experimental samples, and not all the common reference genes are suitable for different tissue samples and/or experimental conditions. In this study, we present the first data of reference genes selection for
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