ArticleBiological procedures online2018
Method for improving the quality of genomic DNA obtained from minute quantities of tissue and blood samples using Chelex 100 resin.
Article in Biological procedures online, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed.
- Review
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- Sexual size dimorphism and morphological sex determination in the Yellow-browed Bunting (Emberiza chrysophrys).BMC zoology · 2025Article
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- Scalable and cost-effective methods for xenomonitoring of P. falciparum and antimalarial drug resistance validated with laboratory and wild-caught mosquitoes.Scientific reports · 2025Article
- A Quantitative Polymerase Chain Reaction Protocol for Sex Identification of Zebra Finch and Chicken Using Blood Samples.bioRxiv : the preprint server for biology · 2025Article
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- Intra- and interspecific variation of Amblyomma ticks from southern Africa.Parasites & vectors · 2024Article
- High prevalence and risk factors associated with asymptomatic malaria among children in Nkwen village, Northwest Region, Cameroon.Malaria journal · 2024Article
- Optimization of Binding Buffer Composition (Polyethylene Glycol, Sodium Chloride and pH) for Extraction of DNA from Biological Fluids Using Polyethyleneimine Functionalized Iron Oxide Nanoparticle-Based Method.Nanotechnology, science and applications · 2024Article
- SURE gel electrophoresis: A method for improved detection and purification of dilute nucleic acid samples.Analytical biochemistry · 2024Article
- Changes in the dopaminergic circuitry and adult neurogenesis linked to reinforcement learning in corvids.Frontiers in neuroscience · 2024Article
- Enhancing malaria detection in resource-limited areas: A high-performance colorimetric LAMP assay for Plasmodium falciparum screening.PloS one · 2024Article
- A rapid and inexpensive 96-well DNA-extraction method from blood using silicon dioxide powder (Glassmilk).Biology methods & protocols · 2024Article
- Antimicrobial resistance and molecular characterization ofVeterinary world · 2023Article
- Antimicrobial resistance and molecular characterization ofJournal of advanced veterinary and animal research · 2023Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough genomic DNA isolation using the Chelex 100 resin is rapid and inexpensive, the DNA obtained by this method has a low concentration in solution and contains suspended impurities. The presence of debris in the DNA solution may result in degradation of DNA on long term storage and inhibition of the polymerase chain reaction. In order to remove impurities and concentrate the DNA in solution, we have introduced modifications in the existing DNA isolation protocol using Chelex-100. We used ammonium acetate to precipitate proteins and a sodium acetate- isopropanol mixture to pellet out DNA which was washed with ethanol.
resultsA pure DNA pellet that can be dissolved in water or Tris-EDTA buffer and stored for a long time at - 80 °C was obtained. We also observed a 20-fold change in the DNA concentration following precipitation and re-dissolution.
conclusionOur method is different from other extraction methods since it uses non-toxic, easily available and inexpensive reagents as well as minimal amounts of blood or tissue samples for the DNA extraction process. Besides its use in sex determination and genotyping in lab animals as described in this paper, it may also have applications in forensic science and diagnostics such as the easy detection of pathogenic DNA in blood.
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