Evidence map›Paper›PMID 29880891›Full record

ArticleScientific reports2018

Snail knockdown reverses stemness and inhibits tumour growth in ovarian cancer.

N Hojo, A L Huisken, H Wang, E Chirshev, N S Kim, S M Nguyen, H Campos, C A Glackin, Y J Ioffe, J J Unternaehrer

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed, 1 pooled it
3.3field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 1 synthesis or guideline pooled it, 76 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

N HojoDivision of Biochemistry, Department of Basic Sciences, Loma Linda University, Loma Linda, CA, USA.
A L HuiskenDivision of Biochemistry, Department of Basic Sciences, Loma Linda University, Loma Linda, CA, USA.
H WangDivision of Biochemistry, Department of Basic Sciences, Loma Linda University, Loma Linda, CA, USA.
E ChirshevDivision of Biochemistry, Department of Basic Sciences, Loma Linda University, Loma Linda, CA, USA.ORCID 0000-0002-3301-2724
N S KimDepartment of Molecular Biology, Chonbuk National University, Dukjindong 664-14, Jeonju, Jeollabuk-do, 561-756, Republic of Korea.
S M NguyenUniversity of California, Riverside - School of Medicine, Riverside, CA, USA.
H CamposDivision of Biochemistry, Department of Basic Sciences, Loma Linda University, Loma Linda, CA, USA.
C A GlackinBeckman Research Institute, City of Hope, Duarte, CA, USA.
Y J IoffeDivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, Loma Linda University Medical Center, Loma Linda, CA, USA.
J J UnternaehrerDivision of Biochemistry, Department of Basic Sciences, Loma Linda University, Loma Linda, CA, USA. junternaehrer@llu.edu.
Loma Linda University · USCity of Hope · USJeonbuk National University · KRLoma Linda University Medical Center · USUniversity of California, Riverside · US

Funding

CIHR
6 · The paper itself

Abstract

To develop effective therapies for advanced high grade serous ovarian cancer (HGSOC), understanding mechanisms of recurrence and metastasis is necessary. In this study, we define the epithelial/mesenchymal status of cell lines that accurately model HGSOC, and evaluate the therapeutic potential of targeting Snai1 (Snail), a master regulator of the epithelial/mesenchymal transition (EMT) in vitro and in vivo. The ratio of Snail to E-cadherin (S/E index) at RNA and protein levels was correlated with mesenchymal morphology in four cell lines. The cell lines with high S/E index (OVCAR8 and COV318) showed more CSC-like, motile, and chemoresistant phenotypes than those with low S/E index (OVSAHO and Kuramochi). We tested the role of Snail in regulation of malignant phenotypes including stemness, cell motility, and chemotherapy resistance: shRNA-mediated knockdown of Snail reversed these malignant phenotypes. Interestingly, the expression of let-7 tumour suppressor miRNA was upregulated in Snail knockdown cells. Furthermore, knockdown of Snail decreased tumour burden in an orthotopic xenograft mouse model. We conclude that Snail is important in controlling HGSOC malignant phenotypes and suggest that the Snail/Let-7 axis may be an attractive target for HGSOC treatment.

Indexed as

Epithelial-Mesenchymal TransitionNeoplasms, ExperimentalNeoplastic Stem CellsOvarian NeoplasmsAnimalsCell Line, TumorFemaleGene Knockdown TechniquesHeterograftsHumansMice, NudeMicroRNAsNeoplasm ProteinsRNA, NeoplasmSnail Family Transcription FactorsMicroRNAsmirnlet7 microRNA, humanNeoplasm ProteinsRNA, NeoplasmSNAI1 protein, humanSnail Family Transcription Factors

Identifiers

PMID29880891
PMCPMC5992154
OpenAlexW2806945910

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.