ArticleBioengineering (Basel, Switzerland)2018
Bioengineering of a Full-Thickness Skin Equivalent in a 96-Well Insert Format for Substance Permeation Studies and Organ-On-A-Chip Applications.
Article in Bioengineering (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- An integrated design strategy for structurally stable full-thickness human skin models with regulatory-qualified barrier assessment.Materials today. Bio · 2026Article
- Recent advances in immunocompetent human skin-on-chip models: Construction strategies and biomedical applications.Bioactive materials · 2026Review
- Microphysiological Systems for Comorbidity Studies: Chronic Kidney Disease and Osteoarthritis.Advanced healthcare materials · 2025Review
- Exploring Multi-Organ Crosstalk via the TissUse HUMIMIC Chip System: Lessons Learnt So Far.Biotechnology and bioengineering · 2025Review
- In Vitro Skin Models as Non-Animal Methods for Dermal Drug Development and Safety Assessment.Pharmaceutics · 2025Review
- Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalentsBiomaterials and biosystems · 2022Article
- Generation and evaluation of input values for computational analysis of transport processes within tissue cultures.Engineering in life sciences · 2022Review
- Proof-of-Concept Organ-on-Chip Study: Topical Cinnamaldehyde Exposure of Reconstructed Human Skin with Integrated Neopapillae Cultured under Dynamic Flow.Pharmaceutics · 2022Article
- Modeling an Optimal 3D Skin-on-Chip within Microfluidic Devices for Pharmacological Studies.Pharmaceutics · 2022Review
- Implementing organ-on-chip in a next-generation risk assessment of chemicals: a review.Archives of toxicology · 2022Review
- Effect of Aronia Extract on Collagen Synthesis in Human Skin Cell and Dermal Equivalent.Oxidative medicine and cellular longevity · 2022Article
- Pigmented Full-Thickness Human Skin Model Based on a Fibroblast-Derived Matrix for Long-Term Studies.Tissue engineering. Part C, Methods · 2021Article
- A new microfluidic method enabling the generation of multi-layered tissues-on-chips using skin cells as a proof of concept.Scientific reports · 2021Article
- Patient-Specific Organoid and Organ-on-a-Chip: 3D Cell-Culture Meets 3D Printing and Numerical Simulation.Advanced biology · 2021Review
- Current Update of Collagen Nanomaterials-Fabrication, Characterisation and Its Applications: A Review.Pharmaceutics · 2021Review
- The microfollicle: a model of the human hair follicle for in vitro studies.In vitro cellular & developmental biology. Animal · 2020Article
- Skin Barriers in Dermal Drug Delivery: Which Barriers Have to Be Overcome and How Can We Measure Them?Pharmaceutics · 2020Review
- Microphysiological Systems: Next Generation Systems for Assessing Toxicity and Therapeutic Effects of Nanomaterials.Small methods · 2020Article
- Improvement of a Three-LayeredFrontiers in bioengineering and biotechnology · 2020Article
- Mechanical Stimulation: A Crucial Element of Organ-on-Chip Models.Frontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human skin is involved in protecting the inner body from constant exposure to outer environmental stimuli. There is an evident need to screen for toxicity and the efficacy of drugs and cosmetics applied to the skin. To date, animal studies are still the standard method for substance testing, although they are currently controversially discussed Therefore, the multi-organ chip is an attractive alternative to replace animal testing. The two-organ chip is designed to hold 96-well cell culture inserts (CCIs). Small-sized skin equivalents are needed for this. In this study, full-thickness skin equivalents (ftSEs) were generated successfully inside 96-well CCIs. These skin equivalents developed with in vivo-like histological architecture, with normal differentiation marker expressions and proliferation rates. The 96-well CCI-based ftSEs were successfully integrated into the two-organ chip. The permeation of fluorescein sodium salt through the ftSEs was monitored during the culture. The results show a decreasing value for the permeation over time, which seems a promising method to track the development of the ftSEs. Additionally, the permeation was implemented in a computational fluid dynamics simulation, as a tool to predict results in long-term experiments. The advantage of these ftSEs is the reduced need for cells and substances, which makes them more suitable for high throughput assays.
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