Evidence map›Paper›PMID 29880030›Full record

ArticleBMC research notes2018

Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation.

Ira M Sigar, Justin H Schripsema, Kathleen A Kelly, Ashlesh K Murthy, Srikanth Manam, Kyle H Ramsey

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ira M SigarMicrobiology and Immunology Department, Chicago College of Osteopathic Medicine, Midwestern University, SH-323H, 555 31st Street, Downers Grove, IL, USA. isigar@midwestern.edu.
Justin H SchripsemaMicrobiology and Immunology Department, Chicago College of Osteopathic Medicine, Midwestern University, SH-323H, 555 31st Street, Downers Grove, IL, USA.
Kathleen A KellyPathology and Laboratory Medicine, David Geffen School of Medicine, at UCLA, Los Angeles, CA, USA.
Ashlesh K MurthyCollege of Veterinary Medicine, Midwestern University, Glendale, AZ, USA.
Srikanth ManamCollege of Veterinary Medicine, Midwestern University, Glendale, AZ, USA.
Kyle H RamseyMicrobiology and Immunology Department, Chicago College of Osteopathic Medicine, Midwestern University, SH-323H, 555 31st Street, Downers Grove, IL, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThis study aims to eliminate Mycoplasma spp. contamination from laboratory stocks of Chlamydia spp. by in vivo passage or by plaque assay.

resultsWe have described two methods of eliminating Mycoplasma contamination from Chlamydia laboratory stocks. We conclude that Mycoplasma species commonly contaminating chlamydial stocks do not survive passage in mice. Chlamydia may also be derived Mycoplasma-free by plaque assay.

Indexed as

ChlamydiaGenetic TechniquesMycoplasmaAnimalsFemaleHeLa CellsHumansMiceMice, Inbred BALB CMice, Inbred C3HMicrobiological TechniquesPolymerase Chain ReactionCell cultureChlamydiaContaminationMycoplasmaPlaque assay

Identifiers

PMID29880030
PMCPMC5992720

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.