ArticleStem cell research2018
Efficient and high yield isolation of myoblasts from skeletal muscle.
Article in Stem cell research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed.
- Peucedanum japonicum Thunb. ameliorates age-related muscle loss by improving mitochondrial function in aged mice.GeroScience · 2026Article
- Prolonged tadalafil exposure promotes skeletal muscle myogenesis and regulates early myogenic markers activation in C2C12 cell lines.Molecular biology reports · 2026Article
- Engineered muscle drives bone repair and functional healing after composite musculoskeletal injury.Biomaterials · 2026Article
- Transwell membrane material affects myogenic differentiation in human primary myoblast-adipocyte co-culture.Physiological reports · 2026Article
- Picalm coordinates clathrin-mediated endocytosis and actin remodeling during myogenesis.Molecular metabolism · 2026Article
- In Vitro Fish Cell Culture: From Primary Muscle Cells to Cell-Based Meat in Cyprinidae.Biology · 2026Review
- Identification of skeletal muscle stem cell adhesion motifs using spot-synthesis-based peptide arrays.iScience · 2026Article
- Bioinspired hydrogel-based regenerative engineering strategies for the structural and functional regeneration of skeletal muscles following volumetric muscle loss.Regenerative biomaterials · 2026Review
- Chick embryo extract-driven culture system enhances chicken muscle satellite cell differentiation and 3D myotube formation.NPJ science of food · 2025Article
- Shear-Induced Patterning of Decellularized Skeletal Muscle Extracellular Matrix for Enhanced Myogenesis.Advanced healthcare materials · 2025Article
- From Development to Regeneration: Insights into Flight Muscle Adaptations from Bat Muscle Cell Lines.Cells · 2025Article
- MOFs-Combining Fully Synthetic Injectable Hydrogel Scaffolds Exhibiting Higher Skeletal Muscle Regenerative Efficiency than Matrigel.Gels (Basel, Switzerland) · 2025Article
- A novel protocol for the direct isolation of a highly pure and regenerative population of satellite stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Macrophage exosomes mediate palmitic acid-induced metainflammation by transferring miR-3064-5p to target IκBα and activate NF-κB signaling.Journal of advanced research · 2025Article
- Quercetin prevents sarcopenia by reversing oxidative stress and mitochondrial damage.Journal of molecular histology · 2025Article
- Stem Cells Associated with Adult Skeletal Muscle Can Form Beating Cardiac Tissue In Vitro in Response to Media Containing Heparin, Dexamethasone, Growth Factors and Hydrogen Peroxide.International journal of molecular sciences · 2025Article
- Zebrafish and cellular models of SELENON-Congenital myopathy exhibit novel embryonic and metabolic phenotypes.Skeletal muscle · 2025Article
- A sort and sequence approach to dissect heterogeneity of response to a self-amplifying RNA vector in a novel human muscle cell line.Molecular therapy. Nucleic acids · 2025Article
- An Efficient Method for Enrichment and In Vitro Propagation of Muscle Stem Cells Derived from Black Sea Bream (Acanthopagrus schlegelii) Skeletal Muscle.Marine biotechnology (New York, N.Y.) · 2025Article
- Creating Optimal Western Blot Conditions for OPA1 Isoforms in Skeletal Muscle Cells and Tissue.Current protocols · 2025Article
17 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
Funding
Abstract
Skeletal muscle (SkM) regeneration relies on the activity of myogenic progenitors that reside beneath the basal lamina of myofibers. Here, we describe a protocol for the isolation of the SkM progenitors from young and old mice by exploiting their outgrowth potential from SkM explants on matrigel coated dishes in the presence of high serum, chicken embryo extract and basic fibroblast growth factor. Compared to other protocols, this method yields a higher number of myoblasts (10-20 million) by enabling the outgrowth of these cells from tissue fragments. The majority of outgrowth cells (~90%) were positive for myogenic markers such as α7-integrin, MyoD, and Desmin. The myogenic cell population could be purified to 98% with one round of pre-plating on collagen coated dishes, where differential attachment of fibroblasts and other non-myogenic progenitors separates them from myoblasts. Moreover, the combination of high serum medium and matrigel coating provided a proliferation advantage to myogenic cells, which expanded rapidly (~24 h population doubling), while non-myogenic cells diminished over time, thereby eliminating the need for further purification steps such as FACS sorting. Finally, myogenic progenitors gave rise to multinucleated myotubes that exhibited sarcomeres and spontaneous beating in the culture dish.
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