ArticleActa biomaterialia2018
Accelerated neural differentiation of mouse embryonic stem cells on aligned GYIGSR-functionalized nanofibers.
Article in Acta biomaterialia, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- The effect of nanomaterials on embryonic stem cell neural differentiation: a systematic review.European journal of medical research · 2023Pooled it
- An innovative treatment for hypoxic-ischemic encephalopathy: Silk fibroin nanomaterials improve neural stem cell axon formation and facilitate cognitive improvement.Neural regeneration research · 2026Article
- PIEZO1-mediated mechanotransduction regulates collagen synthesis on nanostructured 2D and 3D models of fibrosis.Acta biomaterialia · 2025Article
- Rational design matrix materials for organoid development and application in biomedicine.Regenerative biomaterials · 2025Review
- Nanotherapeutic and Stem Cell Therapeutic Strategies in Neurodegenerative Diseases: A Promising Therapeutic Approach.International journal of nanomedicine · 2023Review
- Systematic Alignment Analysis of Neural Transplant Cells in Electrospun Nanofibre Scaffolds.Materials (Basel, Switzerland) · 2022Article
- Article
- Electrospun Fibers Derived from Peptide Coupled Amphiphilic Copolymers for Dorsal Root Ganglion (DRG) Outgrowth.Gels (Basel, Switzerland) · 2021Article
- Tailoring Nano-Porous Surface of Aligned Electrospun Poly (L-Lactic Acid) Fibers for Nerve Tissue Engineering.International journal of molecular sciences · 2021Article
- Electrical Stimulation Promotes Stem Cell Neural Differentiation in Tissue Engineering.Stem cells international · 2021Review
- Polymeric Fibers as Scaffolds for Spinal Cord Injury: A Systematic Review.Frontiers in bioengineering and biotechnology · 2021Review
- Maneuvering the Migration and Differentiation of Stem Cells with Electrospun Nanofibers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Review
- Synergistic Effect of Cell-Derived Extracellular Matrices and Topography on Osteogenesis of Mesenchymal Stem Cells.ACS applied materials & interfaces · 2020Article
- On the Interaction between 1D Materials and Living Cells.Journal of functional biomaterials · 2020Review
- PVDF Nanofiber Scaffold Coated with a Vitronectin Peptide Facilitates the Neural Differentiation of Human Embryonic Stem Cells.Development & reproduction · 2020Article
- Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications-A Review.Polymers · 2020Review
- Functionalization of Electrospun Nanofibers and Fiber Alignment Enhance Neural Stem Cell Proliferation and Neuronal Differentiation.Frontiers in bioengineering and biotechnology · 2020Article
- Postfabrication Tethering of Molecular Gradients on Aligned Nanofibers of Functional Poly(ε-caprolactone)s.Biomacromolecules · 2019Article
- Engineered materials for organoid systems.Nature reviews. Materials · 2019Article
- RGD-Modified Nanofibers Enhance Outcomes in Rats after Sciatic Nerve Injury.Journal of functional biomaterials · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Substrates for embryonic stem cell culture are typified by poorly defined xenogenic, whole proteins or cellular components that are difficult and expensive to generate, characterize, and recapitulate. Herein, the generation of well-defined scaffolds of Gly-Tyr-Ile-Gly-Ser-Arg (GYIGSR) peptide-functionalized poly(ε-caprolactone) (PCL) aligned nanofibers are used to accelerate the neural lineage commitment and differentiation of D3 mouse embryonic stem cells (mESCs). Gene expression trends and immunocytochemistry analysis were similar to laminin-coated glass, and indicated an earlier differentiation progression than D3 mESCs on laminin. Further, GYIGSR-functionalized nanofiber substrates yielded an increased gene expression of Sox1, a neural progenitor cell marker, and Tubb3, Cdh2, Syp, neuronal cell markers, at early time points. In addition, guidance of neurites was found to parallel the fiber direction. We demonstrate the fabrication of a well-defined, xeno-free functional nanofiber scaffold and demonstrates its use as a surrogate for xenogenic and complex matrixes currently used for the neural differentiation of stem cells ex vivo. STATEMENT OF SIGNIFICANCE: In this paper, we report the use of GYIGSR-functionalized poly(ε-caprolactone) aligned nanofibers as a tool to accelerate the neural lineage commitment and differentiation of D3 mouse embryonic stem cells. The results indicate that functional nanofiber substrates promote faster differentiation than laminin coated substrates. The data suggest that aligned nanofibers and post-electrospinning surface modification with bioactive species can be combined to produce translationally relevant xeno-free substrates for stem cell therapy. Future development efforts are focused on additional bioactive species that are able to function as surrogates for other xenogenic factors found in differentiation media.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.