Evidence map›Paper›PMID 29879499›Full record

ArticleGene2018

Generation of a recombinant Aggregatibacter actinomycetemcomitans RTX toxin in Escherichia coli.

Nataliya Balashova, Alexander Giannakakis, Angela C Brown, Evan Koufos, Roland Benz, Tsutomu Arakawa, Hsin-Yao Tang, Edward T Lally

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gene, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Pathogens (Basel, Switzerland) · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 2 countries.

Nataliya BalashovaDepartment of Pathology, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Alexander GiannakakisDepartment of Pathology, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Angela C BrownDepartment of Chemical and Biomolecular Engineering, Lehigh University, Bethlehem, PA, USA.
Evan KoufosDepartment of Chemical and Biomolecular Engineering, Lehigh University, Bethlehem, PA, USA.
Roland BenzDepartment of Life Science and Chemistry, Jacobs University Bremen, Bremen, Germany.
Tsutomu ArakawaAlliance Protein Laboratories, a Division of KBI Biopharma, San Diego, CA, USA.
Hsin-Yao TangProteomics and Metabolomics Facility, The Wistar Institute, Philadelphia, PA, USA.
Edward T LallyDepartment of Pathology, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA. Electronic address: lally@upenn.edu.
University of Pennsylvania · USLehigh University · USAlliance Protein Laboratories (United States) · USConstructor University · DEThe Wistar Institute · US

Funding

VIRULENCE FACTORS IN PERIODONTAL DISEASER01DE009517 · NIDCR · UNIVERSITY OF PENNSYLVANIA · PI BALASHOVA, NATALIYA V · 1990 to 2018
$5.2M
Mechanism of LtxA Enrichment in A. Actinomycetemcomitans Outer Membrane VesiclesR21DE027769 · NIDCR · LEHIGH UNIVERSITY · PI BROWN, ANGELA C. · 2018 to 2019
$426k
NIDCR NIH HHS R01 DE009517NIDCR NIH HHS R21 DE027769
6 · The paper itself

Abstract

A leukotoxin (LtxA) that is produced by Aggregatibacter actinomycetemcomitans (Aa) is an important virulence determinant in an aggressive form of periodontitis in adolescents. Understanding the function of this protein at the molecular level is critical to elucidating its role in the disease process. To accomplish genetic analysis of the protein structure and relating these observations to toxin function, we have developed an E. coli expression system for the generation and rapid purification of LtxA. Cloning the structural toxin gene, ltxA, from Aa strain JP2 under control of T7 promoter-1 of pCDFDuet-1 vector resulted in expression of a 114 KDa protein which could be easily purified by the presence of a carboxy-terminal engineered double hexahistidine (double-His

Indexed as

Aggregatibacter actinomycetemcomitansBacterial ToxinsCloning, MolecularEscherichia coliExotoxinsHumansProtein Structure, SecondaryRecombinant ProteinsTHP-1 CellsBacterial ToxinsExotoxinsleukotoxinRecombinant ProteinsAggregatibacter actinomycetemcomitansLocalized aggressive periodontitisPolyhistidine tagRecombinant toxinRTX toxin

Identifiers

PMID29879499
PMCPMC6640136
OpenAlexW2806778949

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.