ArticleThe Journal of veterinary medical science2018
Development of PCR for identifying Streptococcus parasuis, a close relative of Streptococcus suis.
Article in The Journal of veterinary medical science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Developing a cps-genotyping scheme for the emerging zoonotic pathogen Streptococcus parasuis through genetic analysis of capsular polysaccharide synthesis gene loci.BMC microbiology · 2026Article
- Characterization of a novel gene, Lsa(F), conferring resistance to pleuromutilins, lincosamides and streptogramin A in Streptococcus parasuis.Veterinary research · 2026Article
- First Human Case ofChina CDC weekly · 2026Article
- Developing genome typing strategies for the emerging zoonotic pathogenJournal of clinical microbiology · 2025Article
- Human Streptococcus suis Infections, South America, 1995-2024.Emerging infectious diseases · 2025Review
- Genomic Characterization of Potential Opportunistic ZoonoticPathogens (Basel, Switzerland) · 2025Article
- The first isolation of Clostridium massiliodielmoense from a dead beef cow in Japan and pitfalls to be aware of in identifying the Clostridium species.The Journal of veterinary medical science · 2024Article
- Epidemiology and genetic diversity of Streptococcus suis in smallhold swine farms in the Philippines.Scientific reports · 2023Article
- Co-Existence of Oxazolidinone Resistance GenesAntibiotics (Basel, Switzerland) · 2023Article
- Article
- Complete Genome Sequences of Four Streptococcus parasuis Strains Obtained from Saliva of Domestic Pigs in Japan.Microbiology resource announcements · 2022Article
- Investigation of the Genomic and Pathogenic Features of the Potentially ZoonoticPathogens (Basel, Switzerland) · 2021Article
- Draft Genome Sequence of Streptococcus parasuis 4253, the First Available for the Species.Microbiology resource announcements · 2019Article
- Article
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11 authors.
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Abstract
Streptococcus parasuis has recently been removed taxonomically from Streptococcus suis, a zoonotic pathogen. S. parasuis has been detected in healthy pigs and in diseased pigs, which suggests that S. parasuis is involved in the normal microbiota of pigs and has potential pathogenicity. However, the pathogenicity of S. parasuis in pigs is unclear because of the lack of appropriate detection methods that discriminate S. parasuis from S. suis. In this study, we developed a PCR method that is specific for S. parasuis. The detection limit of the PCR was 350 CFU per reaction. Bacteria isolated from the saliva of eight pigs were collected and examined by PCR. Sixty-four isolates positive for PCR were obtained from the samples of all pigs. Thirteen of the 64 isolates were genetically confirmed as S. parasuis, and biologically and biochemically had nearly the same features of known S. parasuis strains, which suggested that strains positive for PCR were S. parasuis. Among the 64 isolates, 28 isolates were serotypes 20, 22, or 26 in the S. suis serotyping scheme. The remaining 36 isolates were untypeable, which suggested the presence of novel serotypes or a capsule-negative form. Therefore, the PCR method described in this study is a useful tool for identifying S. parasuis, and can be used in etiological studies on this bacterium.
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