ArticleJournal of assisted reproduction and genetics2018
Preincubation with glutathione ethyl ester improves the developmental competence of vitrified mouse oocytes.
Article in Journal of assisted reproduction and genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Role of Glutathione in Alleviating Chilling Injury in Bovine Blastocysts: Mitochondrial Restoration and Apoptosis Inhibition.Antioxidants (Basel, Switzerland) · 2026Article
- Effects and Mechanisms of Imperatorin on Vitrified Mouse Oocytes.Animals : an open access journal from MDPI · 2025Article
- In vivo fertilization improved the cryotolerance and developmental ability of vitrified-warmed rat fertilized oocytes.Scientific reports · 2024Article
- Glutathione during Post-Thaw Recovery Culture Can Mitigate Deleterious Impact of Vitrification on Bovine Oocytes.Antioxidants (Basel, Switzerland) · 2022Article
- Exogenous L-Glutathione Improves Vitrification Outcomes in Murine Preimplantation Embryos.Antioxidants (Basel, Switzerland) · 2022Article
- A Proteomic Study of the Effect of N-acetylcysteine on the Regulation of Early Pregnancy in Goats.Animals : an open access journal from MDPI · 2022Article
- Improved developmental potential of mouse vitrified-warmed oocytes achieved by culturing in recovery medium with glutathione ethyl ester (GSH-OEt).Reproductive medicine and biology · 2021Article
- Assessment of Mitochondrial Function and Developmental Potential of Mouse Oocytes after Mitoquinone Supplementation during Vitrification.Journal of the American Association for Laboratory Animal Science : JAALAS · 2021Article
- N-acetyl-L-cysteine Improves the Developmental Competence of Bovine Oocytes and Embryos Cultured In Vitro by Attenuating Oxidative Damage and Apoptosis.Antioxidants (Basel, Switzerland) · 2021Article
- Ceratonia siliqua (Carob) extract improved in vitro development of vitrified-warmed mouse germinal vesicle oocytes: assessment of possible mechanism.Cell and tissue banking · 2021Article
- Mitochondrial Dysfunction and Oxidative Stress Caused by Cryopreservation in Reproductive Cells.Antioxidants (Basel, Switzerland) · 2021Review
- Glutathione Ethyl Ester Protects In VitroInternational journal of molecular sciences · 2020Article
- The Redox Theory of Development.Antioxidants & redox signaling · 2020Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
purposeOocyte vitrification is currently used for human fertility preservation. However, vitrification damage is a problem caused by decreasing ooplasmic levels of glutathione (GSH). The GSH donor glutathione ethyl ester (GSH-OEt) can significantly increase the GSH content in oocytes. However, it is difficult to obtain oocyte from woman. To overcome this, we used mouse oocytes to replace human oocytes as a model of study.
methodsOocytes from B6D2F1 mice were preincubated for 30 min with 2.5 mmol/L GSH-OEt (GSH-OEt group), without GSH-OEt preincubation before vitrification (control vitrification group) or in nonvitrified oocytes (fresh group). After thawing, oocytes were fertilized for evaluating the developmental competence of embryos in vitro and in vivo. Immunofluorescence, Polscope equipment and quantitative reverse transcription polymerase chain reaction (RT-qPCR) were used to analyze damage, including mitochondrial distribution, reactive oxygen species (ROS) levels, spindle morphology, and gene expression levels (Bcl-2, BAX, and MnSOD).
resultsThe rates of fertilization, 3-4 cell, blastocyst formation and expanded blastocysts were significantly higher (p < 0.05) in the GSH-OEt group (90.4%; 91.1%; 88.9% and 63.0%) than in the control (80.0%; 81.4%; 77.7% and 50.5%). Provided embryos overcame the 2-cell block and developed to the blastocyst stage, birth rates of all groups were similar. Vitrification altered mitochondrial distribution, increased ROS levels, and caused abnormal spindle morphology; GSH-OEt preincubation could improve such damage. RT-qPCR showed that the expression of Bcl-2 was lower in the control group compared with the GSH-OEt group; BAX and MnSoD expression levels were higher in the control group than in the GSH-OEt group (p < 0.05).
conclusionsThe beneficial effect of GSH-OEt preincubation occurred before the 2-cell stage.
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