ArticleFrontiers in cellular and infection microbiology2018
Subtyping of Swine Influenza Viruses Using a High-Throughput Real-Time PCR Platform.
Article in Frontiers in cellular and infection microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Systematic Review of New, Enhanced Surveillance Systems and Methodologies for Zoonotic Influenza Viruses in Animals and Human-Animal Interface.Influenza and other respiratory viruses · 2025Pooled it
- Influenza A virus infection dynamics in two sow herds and effects of interventions.Porcine health management · 2026Article
- Advancements in Modern Nucleic Acid-Based Multiplex Testing Methodologies for the Diagnosis of Swine Infectious Diseases.Veterinary sciences · 2025Review
- Macroepidemiological trends of Influenza A virus detection through reverse transcription real-time polymerase chain reaction (RT-rtPCR) in porcine samples in the United States over the last 20 years.Frontiers in veterinary science · 2025Article
- A New Sampling Approach for the Detection of Swine Influenza a Virus on European Sow Farms.Veterinary sciences · 2022Article
- Article
- Design of a High-Throughput Real-Time PCR System for Detection of Bovine Respiratory and Enteric Pathogens.Frontiers in veterinary science · 2021Article
- Article
- Article
- Substantial Antigenic Drift in the Hemagglutinin Protein of Swine Influenza A Viruses.Viruses · 2020Article
- Development of a high-throughput real-time PCR system for detection of enzootic pathogens in pigs.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Article
- Longitudinal field studies reveal early infection and persistence of influenza A virus in piglets despite the presence of maternally derived antibodies.Veterinary research · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Influenza A viruses (IAVs) are important human and animal pathogens with high impact on human and animal health. In Denmark, a passive surveillance program for IAV in pigs has been performed since 2011, where screening tests and subsequent subtyping are performed by reverse transcription quantitative real-time PCR (RT-qPCR). A disadvantage of the current subtyping system is that several assays are needed to cover the wide range of circulating subtypes, which makes the system expensive and time-consuming. Therefore, the aim of the present study was to develop a high-throughput method, which could improve surveillance of swine influenza viruses (swIAVs) and lower the costs of virus subtyping. Twelve qPCR assays specific for various hemagglutinin and neuraminidase gene lineages relevant for swIAV and six assays specific for the internal genes of IAV were developed and optimized for the high-throughput qPCR platform BioMark (Fluidigm). The qPCR assays were validated and optimized to run under the same reaction conditions using a 48.48 dynamic array (48.48DA). The sensitivity and specificity was assessed by testing virus isolates and field samples with known subtypes. The results revealed a performance of the swIAV 48.48DA similar to conventional real-time analysis, and furthermore, the specificity of swIAV 48.48DA was very high and without cross reactions between the assays. This high-throughput system provides a cost-effective alternative for subtyping of swIAVs.
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