Evidence map›Paper›PMID 29872452›Full record

ArticlePlant methods2018

A simple and cost-effective method for screening of CRISPR/Cas9-induced homozygous/biallelic mutants.

Jinggong Guo, Kun Li, Lifeng Jin, Rui Xu, Kaiting Miao, Fengbo Yang, Chaoya Qi, Lin Zhang, Jose R Botella, Ran Wang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Development of the CRISPR-Cas9 System for the Marine-Derived FungiJournal of fungi (Basel, Switzerland) · 2022
    Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. Review
  11. Review
  12. Review
  13. CRISPR/Cas9-Mediated Generation of Pathogen-Resistant Tomato againstInternational journal of molecular sciences · 2021
    Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Jinggong Guo *1State Key Laboratory of Cotton Biology, Department of Biology, Institute of Plant Stress Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.
Kun Li *1State Key Laboratory of Cotton Biology, Department of Biology, Institute of Plant Stress Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.
Lifeng Jin *Zhengzhou Tabacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou, 450001 Henan China.
Rui Xu *1State Key Laboratory of Cotton Biology, Department of Biology, Institute of Plant Stress Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.
Kaiting Miao1State Key Laboratory of Cotton Biology, Department of Biology, Institute of Plant Stress Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.
Fengbo Yang1State Key Laboratory of Cotton Biology, Department of Biology, Institute of Plant Stress Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.
Chaoya QiZhengzhou Tabacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou, 450001 Henan China.
Lin ZhangZhengzhou Tabacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou, 450001 Henan China.
Jose R Botella5School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD Australia.
Ran WangZhengzhou Tabacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou, 450001 Henan China.
Yuchen Miao1State Key Laboratory of Cotton Biology, Department of Biology, Institute of Plant Stress Biology, Henan University, 85 Minglun Street, Kaifeng, 475001 China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe CRISPR/Cas9 system is being used for genome editing purposes by many research groups in multiple plant species. Traditional sequencing methods to identify homozygous mutants are time-consuming, laborious and expensive.

resultsWe have developed a method to screen CRISPR/Cas9-induced mutants through Mutation Sites Based Specific Primers Polymerase Chain Reaction (MSBSP-PCR). The MSBSP-PCR method was successfully used to identify homozygous/biallelic mutants in

conclusionsThe MSBSP-PCR method is simple to implement and can save time and cost in the screening of CRISPR/Cas9-induced homozygous/biallelic mutants.

Indexed as

Arabidopsis thalianaCRISPR/Cas9Genome editingMutant screeningPCRTobacco

Identifiers

PMID29872452
PMCPMC5972395

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.