ArticleScientific reports2018
Prestressed cells are prone to cytoskeleton failures under localized shear strain: an experimental demonstration on muscle precursor cells.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
- Extracellular-matrix mediated stresses spatially bias myoblast fusion and myotube growth.Nature communications · 2026Article
- Activity-dependent glassy cell mechanics Ⅰ: Mechanical properties measured with active microrheology.Biophysical journal · 2023Article
- Quality control methods in musculoskeletal tissue engineering: from imaging to biosensors.Bone research · 2021Review
- Effect of Nuclear Stiffness on Cell Mechanics and Migration of Human Breast Cancer Cells.Frontiers in cell and developmental biology · 2020Article
- Neuronal Plasma Membrane Integrity is Transiently Disturbed by Traumatic Loading.Neuroscience insights · 2020Article
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7 authors.
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Abstract
We report on a wavelet based space-scale decomposition method for analyzing the response of living muscle precursor cells (C2C12 myoblasts and myotubes) upon sharp indentation with an AFM cantilever and quantifying their aptitude to sustain such a local shear strain. Beyond global mechanical parameters which are currently used as markers of cell contractility, we emphasize the necessity of characterizing more closely the local fluctuations of the shear relaxation modulus as they carry important clues about the mechanisms of cytoskeleton strain release. Rupture events encountered during fixed velocity shear strain are interpreted as local disruptions of the actin cytoskeleton structures, the strongest (brittle) ones being produced by the tighter and stiffer stress fibers or actin agglomerates. These local strain induced failures are important characteristics of the resilience of these cells, and their aptitude to maintain their shape via a quick recovery from local strains. This study focuses on the perinuclear region because it can be considered as a master mechanical organizing center of these muscle precursor cells. Using this wavelet-based method, we combine the global and local approaches for a comparative analysis of the mechanical parameters of normal myoblasts, myotubes and myoblasts treated with actomyosin cytoskeleton disruptive agents (ATP depletion, blebbistatin).
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