ArticleScientific reports2018
Comparing Sanger sequencing and high-throughput metabarcoding for inferring photobiont diversity in lichens.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
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28 citing papers in PubMed, 55 citations in OpenAlex.
- The Impact of Visible Symptoms of Thallus Damage on the Phycobiota of Mediterranean Epiphytic Lichens.Microbial ecology · 2026Article
- Chloroplast and Mitochondrial Genomes of the Lichen-Symbiotic Green Alga Trebouxia Illuminate Evolutionary Relationships and Climate Associations and Yield New Phylogenetic Markers.Genome biology and evolution · 2026Article
- Islands of Biodiversity: Characterization of Lichen Flora in Antarctic Nunataks.Journal of fungi (Basel, Switzerland) · 2026Article
- Members of the lichen photobiont genus Trebouxia show species-specific photophysiological and transcriptome-level responses to high light.Journal of experimental botany · 2026Article
- Convergent symbioses: morphology, life history, and niche specialization in coral and lichen mutualisms.FEMS microbiology reviews · 2026Review
- The genome sequence ofWellcome open research · 2026Article
- Multi-platform metagenomic characterization of the microbial community during spontaneous cacao fermentation.Frontiers in bioengineering and biotechnology · 2025Article
- Genome-Wide Comparisons Reveal Extensive Divergence Within the Lichen Photobiont Genus, Trebouxia.Genome biology and evolution · 2024Article
- 16S rDNA Sequencing for Bacterial Identification in Preterm Infants with Suspected Early-Onset Neonatal Sepsis.Tropical medicine and infectious disease · 2024Article
- Distinct orchid mycorrhizal fungal communities among co-occurring Vanilla species in Costa Rica: root substrate and population-based segregation.Mycorrhiza · 2024Article
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- Bacteriomic Profiles of Rock-Dwelling Lichens from the Venezuelan Guiana Shield and the South African Highveld Plateau.Microorganisms · 2024Article
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- Survey of Lichenized Fungi DNA Barcodes on King George Island (Antarctica): An Aid to Species Discovery.Journal of fungi (Basel, Switzerland) · 2023Article
- Biodiversity of Basidiomycetous Yeasts Associated withJournal of fungi (Basel, Switzerland) · 2023Article
- Photobiont Diversity in Lichen Symbioses From Extreme Environments.Frontiers in microbiology · 2022Article
- A Closer Examination of the 'Abundant-Center' for Ectomycorrhizal Fungal Community Associated WithFrontiers in plant science · 2022Article
- Global Biodiversity Patterns of the Photobionts Associated with the Genus Cladonia (Lecanorales, Ascomycota).Microbial ecology · 2021Article
- Microbial Communities ofMicroorganisms · 2021Article
- Turnover of Lecanoroid Mycobionts and TheirFrontiers in microbiology · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The implementation of HTS (high-throughput sequencing) approaches is rapidly changing our understanding of the lichen symbiosis, by uncovering high bacterial and fungal diversity, which is often host-specific. Recently, HTS methods revealed the presence of multiple photobionts inside a single thallus in several lichen species. This differs from Sanger technology, which typically yields a single, unambiguous algal sequence per individual. Here we compared HTS and Sanger methods for estimating the diversity of green algal symbionts within lichen thalli using 240 lichen individuals belonging to two species of lichen-forming fungi. According to HTS data, Sanger technology consistently yielded the most abundant photobiont sequence in the sample. However, if the second most abundant photobiont exceeded 30% of the total HTS reads in a sample, Sanger sequencing generally failed. Our results suggest that most lichen individuals in the two analyzed species, Lasallia hispanica and L. pustulata, indeed contain a single, predominant green algal photobiont. We conclude that Sanger sequencing is a valid approach to detect the dominant photobionts in lichen individuals and populations. We discuss which research areas in lichen ecology and evolution will continue to benefit from Sanger sequencing, and which areas will profit from HTS approaches to assessing symbiont diversity.
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