ArticleBMC plant biology2018
The effect of promoter methylation on MdMYB1 expression determines the level of anthocyanin accumulation in skins of two non-red apple cultivars.
Article in BMC plant biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 80 citations in OpenAlex.
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- Combined analysis of lncRNAs and mRNAs associated with coloration and wax formation during 'Fumei' Apple development.BMC plant biology · 2025Article
- A transcription factor, PbWRKY24, contributes to russet skin formation in pear fruits by modulating lignin accumulation.Horticulture research · 2025Article
- Integrated Metabolomics and Transcriptome Analysis of Anthocyanin Biosynthetic Pathway inPlants (Basel, Switzerland) · 2025Article
- Hypermethylation in the promoter regions of flavonoid pathway genes is associated with skin color fading during 'Daihong' apple fruit development.Horticulture research · 2024Article
- PlgMYBR1, an R2R3-MYB transcription factor, plays as a negative regulator of anthocyanin biosynthesis in3 Biotech · 2023Article
- Transcriptomic analysis reveals biosynthesis genes and transcription factors related to leaf anthocyanin biosynthesis in Aglaonema commutatum.BMC genomics · 2023Article
- Involvement of 5mC DNA demethylationForestry research · 2023Article
- Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells.Genes · 2022Article
- Effect of Paper-Bagging on Apple Skin Patterning Associated withInternational journal of molecular sciences · 2022Article
- Involvement of histone deacetylase CsHDA2 in regulating (Horticulture research · 2022Article
- A naturally-occurring phenomenon of flower color change during flower development inFrontiers in plant science · 2022Article
- Feasible strategies for studying the involvement of DNA methylation and histone acetylation in the stress-induced formation of quality-related metabolites in tea (Camellia sinensis).Horticulture research · 2021Review
- Metabolome and transcriptome profiling provide insights into green apple peel reveals light- and UV-B-responsive pathway in anthocyanins accumulation.BMC plant biology · 2021Article
- Comparative Transcriptome Analysis of DifferentBiology · 2021Article
- Influence of Bagging on the Development and Quality of Fruits.Plants (Basel, Switzerland) · 2021Review
- Genome-wide identification and analysis of DNA methyltransferase and demethylase gene families in Dendrobium officinale reveal their potential functions in polysaccharide accumulation.BMC plant biology · 2021Article
- Article
- Skin Color in Apple Fruit (Epigenomes · 2020Review
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8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundFruit color in apple (Malus domestica Borkh.) is ascribed mainly to the accumulation of anthocyanin pigments, and is an important trait for determining fruit market acceptance. Bagging is a commonly used treatment to enhance the red pigmentation in apple skin. The MdMYB1 transcription factor gene plays an important role in the biosynthesis of anthocyanin in apple after bag removal, but little is known about how MdMYB1 transcription is regulated.
resultsIn this study, we investigated pigmentation in the non-red skinned cultivars 'Granny Smith' and 'Golden Delicious' after bag removal. The fruit skins of the two cultivars showed red/pink pigmentation after bag treatment. Transcript levels of MdMYB1, the master regulator of anthocyanin biosynthesis in apple, increased, and showed a correlation with anthocyanin content in both cultivars after bag removal. The MdMYB1 genomic sequences were compared in the two cultivars, which showed that the green-fruited cultivar 'Granny Smith' harbors the MdMYB1-1 and MdMYB1-2 alleles, while the yellow-fruited cultivar 'Golden Delicious' harbors only MdMYB1-2. A comparison of methylation levels in the 2 kb region upstream of the MdMYB1 ATG between the bag-treated fruits after removal from the bags and the unbagged fruits showed a correlation between hypomethylation and the red-skin phenotype in 'Granny Smith'. Moreover, 'Granny Smith' fruits responded to treatment with 5-aza-2'-deoxycytidine, an inducer of DNA demethylation. An investigation of the MdMYB1 promoter in 'Granny Smith' showed reduced methylation in the regions - 2026 to - 1870 bp, - 1898 to - 1633 bp, and - 541 to - 435 bp after bag removal and 5-aza-2'-deoxycytidine treatments.
conclusionsDifferences in anthocyanin levels between 'Granny Smith' and 'Golden Delicious' can be explained by differential accumulation of MdMYB1-specific mRNA. Different levels of MdMYB1 transcripts in the two cultivars are associated with methylation levels in the promoter region. Hypomethylation of the MdMYB1 promoter is correlated with the formation of red pigmentation in 'Granny Smith' fruit skins. As a result, red pigmentation in Granny Smith' was more intense than in 'Golden Delicious' fruits after bag removal.
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