ArticleBMC infectious diseases2018
Brucella abortus: determination of survival times and evaluation of methods for detection in several matrices.
Article in BMC infectious diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Development and evaluation of a novel lateral flow immunoassay for rapid diagnosis of brucellosis across different animal species.Scientific reports · 2025Article
- Enhanced Isolation ofMicroorganisms · 2025Article
- Risk Assessment ofVeterinary sciences · 2025Article
- A detailed review of bovine brucellosis.Open veterinary journal · 2025Review
- Specific detection of Brucella spp. from slaughter-aged livestock using a dual priming oligonucleotide system.BMC microbiology · 2025Article
- Preparation and application of specific chicken yolk antibodies in detectingFrontiers in veterinary science · 2025Article
- Molecular and serological diagnosis of multiple bacterial zoonoses in febrile outpatients in Garissa County, north-eastern Kenya.Scientific reports · 2024Article
- Serological, phenotypic and molecular characterization of brucellosis in small ruminants in northern Algeria.Frontiers in microbiology · 2024Article
- Brucellosis of the spine - A global public health problem - An analysis of 37 patients from a 'high risk' region.Journal of clinical orthopaedics and trauma · 2023Article
- Disease Occurrence in- and the Transferal of Zoonotic Agents by North American Feedlot Cattle.Foods (Basel, Switzerland) · 2023Review
- Evaluation of a Real-time PCR in Combination with a Cultivation Method for the Detection ofJournal of veterinary research · 2022Article
- Another lesson from unmapped reads: in-depth analysis of RNA-Seq reads from various horse tissues.Journal of applied genetics · 2022Article
- Evidence of co-exposure with Brucella spp, Coxiella burnetii, and Rift Valley fever virus among various species of wildlife in Kenya.PLoS neglected tropical diseases · 2022Article
- Article
- The Importance of Complementary PCR Analysis in Addition to Serological Testing for the Detection of Transmission Sources ofVeterinary sciences · 2022Article
- Molecular detection of novelOpen research Africa · 2022Article
- The Use of Flocked Swabs with a Protective Medium Increases the Recovery of Live Brucella spp. and DNA Detection.Microbiology spectrum · 2021Article
- Importance of brucellosis control programs of livestock on the improvement of one health.The veterinary quarterly · 2021Review
- Brucellosis of the Spine in Children-An Analysis of 5 Children From an Endemic Setting.International journal of spine surgery · 2021Article
- Risk Factors for Acute Brucellosis in Patients on the Day of Admission at Selected Hospitals of Abbottabad, Pakistan.Frontiers in public health · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundBrucella abortus is a highly pathogenic zoonotic agent, tempting for the development of a rapid diagnostic method to enable adequate treatment and prevent further spread. Enrichment of the bacteria is often used as a first step in diagnostics to increase the bacterial number above the detection limit of the real-time PCR. The enrichment of Brucella spp. takes at least 3 days, which might be avoidable if sensitive PCR methods can be used. Since many matrices contain PCR inhibitors, the limit of detection (LOD) must be determined for each separate matrix. Another aim of this study was the determination of survival of Brucella abortus in the analyzed matrices.
methodsThe LOD for the detection of B. abortus in 14 matrices, relevant for human medicine, veterinary medicine and food and feed safety, was determined to evaluate the need of a pre-enrichment step prior to real-time PCR. The survival of B. abortus in the spiked matrices was tested by plate count in a 7-day interval for 132 days.
resultsThe limit of detection for B. abortus in most matrices was in the range of 10
conclusionsA direct PCR analysis without enrichment of bacteria saves at least 3 days. However, the limit of detection between direct PCR and plate count differs in a 10 fold range. We conclude that this lower sensitivity is acceptable in most cases especially if quick analysis are required.
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