Evidence map›Paper›PMID 29870994›Full record

ArticleKidney & blood pressure research2018

Enhanced Renal Afferent Arteriolar Reactive Oxygen Species and Contractility to Endothelin-1 Are Associated with Canonical Wnt Signaling in Diabetic Mice.

Suping Zhang, Qian Huang, Qiaoling Wang, Qin Wang, Xiaoyun Cao, Liang Zhao, Nan Xu, Zhengbing Zhuge, Jianhua Mao, Xiaodong Fu and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Kidney & blood pressure research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 6 institutions in 3 countries.

Suping ZhangDepartment of Physiology, and the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Qian HuangDepartment of Physiology, and the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Qiaoling WangDepartment of Physiology, and the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Qin WangDepartment of Physiology, and the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Xiaoyun CaoDepartment of Physiology, and the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Liang ZhaoDepartment of Physiology, and the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Nan XuDepartment of Physiology, and the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Zhengbing ZhugeDepartment of Physiology, and the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Jianhua MaoDepartment of Physiology, and the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Xiaodong FuDepartment of Physiology, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China.
Ruisheng LiuDepartment of Molecular Pharmacology & Physiology, University of South Florida College of Medicine, Tampa, Florida, USA.
Christopher S WilcoxDivision of Nephrology and Hypertension, and Hypertension Center, Georgetown University, Washington, District of Columbia, USA.
Andreas PatzakInstitute of Vegetative Physiology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Lingli LiDivision of Nephrology and Hypertension, and Hypertension Center, Georgetown University, Washington, District of Columbia, USA.
En Yin LaiDepartment of Physiology, and the Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China, laienyin@zju.edu.cn.
Children's Hospital of Zhejiang University · CNGeorgetown University · USGuangzhou Medical University · CNCharité - Universitätsmedizin Berlin · DEQuanzhou Normal University · CNUniversity of South Florida · US

Funding

Tubuloglomerular feedback and salt-sensitive hypertensionR01DK098582 · NIDDK · UNIVERSITY OF SOUTH FLORIDA · PI LIU, RUISHENG · 2013 to 2016
$1.3M
Primary cilia and modulation of the renal microcirculationR01DK099276 · NIDDK · UNIVERSITY OF SOUTH FLORIDA · PI LIU, RUISHENG · 2014 to 2017
$980k
NIDDK NIH HHS R01 DK098582NIDDK NIH HHS R01 DK099276
6 · The paper itself

Abstract

BACKGROUND/

aimsCanonical Wnt signaling is involved in oxidative stress, vasculopathy and diabetes mellitus but its role in diabetic renal microvascular dysfunction is unclear. We tested the hypothesis that enhanced canonical Wnt signaling in renal afferent arterioles from diabetic mice increases reactive oxygen species (ROS) and contractions to endothelin-1 (ET-1).

methodsStreptozotocin-induced diabetes or control C57Bl/6 mice received vehicle or sulindac (40 mg·kg-1·day-1) to block Wnt signaling for 4 weeks. ET-1 contractions were measured by changes of afferent arteriolar diameter. Arteriolar H2O2, O2 -, protein expression and enzymatic activity were assessed using sensitive fluorescence probes, immunoblotting and colorimetric assay separately.

resultsCompared to control, diabetic mouse afferent arteriole had increased O2- (+ 84%) and H2O2 (+ 91%) and enhanced responses to ET-1 at 10-8 mol·l-1 (-72±4% of versus -43±4%, P< 0.05) accompanied by reduced protein expressions and activities for catalase and superoxide dismutase 2 (SOD2). Arteriolar O2 - was increased further by ET-1 and contractions to ET-1 reduced by PEG-SOD in both groups whereas H2O2 unchanged by ET-1 and contractions were reduced by PEG-catalase selectively in diabetic mice. The Wnt signaling protein β-catenin was upregulated (3.3-fold decrease in p-β-catenin/β-catenin) while the glycogen synthase kinase-3β (GSK-3β) was downregulated (2.6-fold increase in p-GSK-3β/ GSK-3β) in preglomerular vessels of diabetic mice. Sulindac normalized the Wnt signaling proteins, arteriolar O2 -, H2O2 and ET-1 contractions while doubling microvascular catalase and SOD2 expression in diabetic mice.

conclusionIncreased ROS, notably H2O2 contributes to enhanced afferent arteriolar responses to ET-1 in diabetes, which is closely associated with Wnt signaling. Antioxidant pharmacological strategies targeting Wnt signaling may improve vascular function in diabetic nephropathy.

Indexed as

Wnt Signaling PathwayAnimalsArteriolesDiabetes Mellitus, ExperimentalEndothelin-1Hydrogen PeroxideKidneyMiceReactive Oxygen SpeciesVasoconstrictionEndothelin-1Hydrogen PeroxideReactive Oxygen SpeciesAfferent arterioleCanonical Wnt signalingDiabetes mellitusEndothelin-1Reactive oxygen species

Identifiers

PMID29870994
PMCPMC6050514
OpenAlexW2805996934

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.