Evidence map›Paper›PMID 29870526›Full record

ArticlePloS one2018

The expression of telomere-related proteins and DNA damage response and their association with telomere length in colorectal cancer in Saudi patients.

Ftoon Aljarbou, Nourah Almousa, Mohammad Bazzi, Sooad Aldaihan, Mohammed Alanazi, Othman Alharbi, Majid Almadi, Abdulrahman M Aljebreen, Nahla Ali Azzam, Maha Arafa and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Role of telomere length in human carcinogenesis (Review).International journal of oncology · 2023
    Review
  3. REIA: A database for cancer A-to-I RNA editing with interactive analysis.International journal of biological sciences · 2022
    Article
  4. Review
  5. Non-canonical roles of canonical telomere binding proteins in cancers.Cellular and molecular life sciences : CMLS · 2021
    Review
  6. Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 1 institution in 1 country.

Ftoon AljarbouGenome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.ORCID 0000-0001-6859-638X
Nourah AlmousaGenome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Mohammad BazziGenome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Sooad AldaihanGenome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Mohammed AlanaziGenome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Othman AlharbiDivision of Gastroenterology, King Khalid University Hospital, King Saud University, Riyadh, Saudi Arabia.
Majid AlmadiDivision of Gastroenterology, King Khalid University Hospital, King Saud University, Riyadh, Saudi Arabia.
Abdulrahman M AljebreenDivision of Gastroenterology, King Khalid University Hospital, King Saud University, Riyadh, Saudi Arabia.
Nahla Ali AzzamDivision of Gastroenterology, King Khalid University Hospital, King Saud University, Riyadh, Saudi Arabia.
Maha ArafaDepartment of Histopathology, King Saud University, King Khalid University Hospital, Riyadh, Saudi Arabia.ORCID 0000-0003-4966-2358
Abeer AldbassGenome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Jilani ShaikGenome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Shaheerah AlasirriGenome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Arjumand WarsyGenome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Abdullah AlamriGenome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Narasimha Reddy ParineGenome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Ghadah AlamroGenome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
King Saud University · SA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundColorectal cancer is the leading cause of cancer-related deaths in Saudi Arabia. Cancer has a multifactorial nature and can be described as a disease of altered gene expression. The profiling of gene expression has been used to identify cancer subtypes and to predict patients' responsiveness. Telomere-associated proteins that regulate telomere biology are essential molecules in cancer development. Thus, the present study examined their contributions to colorectal cancer progression in Saudi patients.

methodsThe expression of hTERT, TRF1, TRF2, POT1, ATR, ATM, Chk1 and Chk2 were measured via real-time PCR in matched cancerous and adjacent tissues of CRC patients. The protein level of hTERT, TRF1, TRF2, ATR, ATM, Chk1 and Chk2 were measured using immunohistochemistry. A region of hTERT core promoter was sequenced via Sanger sequencing. Methylation of CTCF binding site was examined via methylation-specific PCR. Finally, the length of telomere was estimated using q-PCR.

resultsOur results showed that POT1, ATR, Chk1 and Chk2 show increased expression in CRC relative to the adjacent mucosa. The expression levels of each gene were associated with clinicopathological characteristics of patients with CRC. There was a positive correlation between the age of the patients and hTERT expression. Regarding tumor site, telomere length, ATR, ATM and Chk1 were shown to be altered. No somatic mutation was detected in hTERT core promoter, and no differences in methylation patterns at CTCF binding site in the promoter between normal and cancer tissues.

conclusionAnalysis of targeted genes expression in colorectal cancer based on the clinical variables revealed that tumor location and age could have a role in gene expression and telomere length variations and this could be taken under consideration during CRC diagnosis and therapy. Other epigenetic mechanisms could influence hTERT expression in cancers. Our findings warrant further validation through experiments involving a larger number of patients.

Indexed as

DNA DamageGene Expression Regulation, NeoplasticTelomere HomeostasisAdultAgedColorectal NeoplasmsFemaleHumansIntestinal MucosaMaleMiddle AgedNeoplasm ProteinsTelomere-Binding ProteinsNeoplasm ProteinsTelomere-Binding Proteins

Identifiers

PMID29870526
PMCPMC5988329
OpenAlexW2805470845

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.