ArticleJournal of the American Chemical Society2018
Macromolecularly Crowded Protocells from Reversibly Shrinking Monodisperse Liposomes.
Article in Journal of the American Chemical Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 98 citations in OpenAlex.
- Tumor-Mimic Artificial Cell Integrated With In Situ Synthetic Biology for Testing of Antitumor Drug Sensitivity.Exploration (Beijing, China) · 2026Article
- Solvent-Mediated Dewetting Principles for Cell-Sized Liposome Formation.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Interfacial assembly of biomimetic MOF-based porous membranes on coacervates to build complex protocells and prototissues.Nature chemistry · 2025Article
- Liposome-EncapsulatedACS synthetic biology · 2025Article
- Challenges in observing transcription-translation for bottom-up synthetic biology.QRB discovery · 2025Review
- Membraneless protocell confined by a heat flow.Nature physics · 2025Article
- Lipase activated endocytosis-like behavior of oil-in-water emulsion.Nature communications · 2024Article
- Polyphenol-stabilized coacervates for enzyme-triggered drug delivery.Nature communications · 2024Article
- Artificial cells for in vivo biomedical applications through red blood cell biomimicry.Nature communications · 2024Review
- DisGUVery: A Versatile Open-Source Software for High-Throughput Image Analysis of Giant Unilamellar Vesicles.ACS synthetic biology · 2023Article
- Compartmentalized Cell-Free Expression Systems for Building Synthetic Cells.Advances in biochemical engineering/biotechnology · 2023Article
- High Macromolecular Crowding in Liposomes from Microfluidics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Triggerable Protocell Capture in Nanoparticle-Caged Coacervate Microdroplets.Journal of the American Chemical Society · 2022Article
- System concentration shift as a regulator of transcription-translation system within liposomes.iScience · 2021Article
- Membrane-confined liquid-liquid phase separation toward artificial organelles.Science advances · 2021Article
- Recent developments in microfluidic synthesis of artificial cell-like polymersomes and liposomes for functional bioreactors.Biomicrofluidics · 2021Article
- Modularize and Unite: Toward Creating a Functional Artificial Cell.Frontiers in molecular biosciences · 2021Review
- Transcription and Translation in Cytomimetic Protocells Perform Most Efficiently at Distinct Macromolecular Crowding Conditions.ACS synthetic biology · 2020Article
- Construction of coacervate-in-coacervate multi-compartment protocells for spatial organization of enzymatic reactions.Chemical science · 2020Article
- Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology.Frontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The compartmentalization of cell-free gene expression systems in liposomes provides an attractive route to the formation of protocells, but these models do not capture the physical (crowded) environment found in living systems. Here, we present a microfluidics-based route to produce monodisperse liposomes that can shrink almost 3 orders of magnitude without compromising their stability. We demonstrate that our strategy is compatible with cell-free gene expression and show increased protein production rates in crowded liposome protocells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.