Crystallographic analysis of the Staphylococcus epidermidis lipase involved in esterification in aqueous solution.

Cheng Huan Liu, Yu Ting Chen, Ming Hon Hou, Nien Jen Hu, Chin Shuh Chen, Jei Fu Shaw

Open access · bronzeAbstract read

In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 96% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Cheng Huan LiuDepartment of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Road, South District, Taichung City 402, Taiwan.

Yu Ting ChenInstitute of Genomics and Bioinformatics, National Chung Hsing University, 145 Xingda Road, South District, Taichung City 402, Taiwan.

Ming Hon HouInstitute of Genomics and Bioinformatics, National Chung Hsing University, 145 Xingda Road, South District, Taichung City 402, Taiwan.

Nien Jen HuInstitute of Biochemistry, National Chung Hsing University, 145 Xingda Road, South District, Taichung City 402, Taiwan.

Chin Shuh ChenDepartment of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Road, South District, Taichung City 402, Taiwan.

Jei Fu ShawDepartment of Biological Science and Technology, I-Shou University, No. 1, Section 1, Syuecheng Road, Dashu District, Kaohsiung City 84001, Taiwan.

National Chung Hsing University · TWI-Shou University · TW

Funding

Ministry of Science and Technology, Taiwan MOST 104-2313-B-005-007-MY2Ministry of Science and Technology, Taiwan MOST 106-2313-B-005-035-MY2

6 · The paper itself

Abstract

The Staphylococcus epidermidis lipase (SeLip, GehC) can be used in flavour-compound production via esterification in aqueous solution. This study reports the crystallization and crystallographic analysis of recombinant GehC (rGehC; Lys303-Lys688) with a molecular weight of 43 kDa. rGehC was crystallized at 293 K using PEG 10 000 as a precipitant, and a 99.9% complete native data set was collected from a cooled crystal at 77 K to a resolution of 1.9 Å with an overall R

Indexed as

WaterAmino Acid SequenceCrystallographyEsterificationLipaseSolutionsStaphylococcus epidermidisLipaseSolutionsWateraqueous mediacatalytic mechanismlipaseSeLipStaphylococcus epidermidis

Identifiers

PMID29870019

DOI10.1107/S2053230X18006775

PMCPMC5987743

OpenAlexW2804080214

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.