ArticleeLife2018
Temporal profiling of redox-dependent heterogeneity in single cells.
Article in eLife, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Coupled intracellular redox and extracellular respiration sensing for quantitative oxidative stress profiling.bioRxiv : the preprint server for biology · 2026Article
- Redox-driven regulation of UCHL3/Yuh1 influences mitochondrial health via the NEDD8/Rub1 pathway.Redox biology · 2025Article
- Impact of Oxygen Availability on the Organelle-Specific Redox Potentials and Stress in Recombinant Protein Producing Komagataella phaffii.Microbial biotechnology · 2025Article
- Fundamentals of redox regulation in biology.Nature reviews. Molecular cell biology · 2024Review
- Article
- Remodeling of the focal adhesion complex by hydrogen-peroxide-induced senescence.Scientific reports · 2023Article
- Real-time redox adaptations in human airway epithelial cells exposed to isoprene hydroxy hydroperoxide.Redox biology · 2023Article
- Exercise-Induced Adipose Tissue Thermogenesis and Browning: How to Explain the Conflicting Findings?International journal of molecular sciences · 2022Review
- Article
- Review
- In Vivo Imaging with Genetically Encoded Redox Biosensors.International journal of molecular sciences · 2020Review
- The Preservative Sorbic Acid Targets Respiration, Explaining the Resistance of Fermentative Spoilage Yeast Species.mSphere · 2020Article
- A tryparedoxin-coupled biosensor reveals a mitochondrial trypanothione metabolism in trypanosomes.eLife · 2020Article
- TrypOx, a Novel Eukaryotic Homolog of the Redox-Regulated Chaperone Hsp33 inFrontiers in microbiology · 2020Article
- Utilizing redox-sensitive GFP fusions to detect in vivo redox changes in a genetically engineered prokaryote.Redox biology · 2019Article
- Article
- Variety is the spice of life: how to explore a redox-dependent heterogeneity in genomically identical cellular populations.Current genetics · 2019Article
- How DoesFrontiers in microbiology · 2019Article
- Proteasome lid bridges mitochondrial stress with Cdc53/Cullin1 NEDDylation status.Redox biology · 2019Article
- High-Reynolds Microfluidic Sorting of Large Yeast Populations.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
14 authors.
Funding
Abstract
Cellular redox status affects diverse cellular functions, including proliferation, protein homeostasis, and aging. Thus, individual differences in redox status can give rise to distinct sub-populations even among cells with identical genetic backgrounds. Here, we have created a novel methodology to track redox status at single cell resolution using the redox-sensitive probe Grx1-roGFP2. Our method allows identification and sorting of sub-populations with different oxidation levels in either the cytosol, mitochondria or peroxisomes. Using this approach, we defined a redox-dependent heterogeneity of yeast cells and characterized growth, as well as proteomic and transcriptomic profiles of distinctive redox subpopulations. We report that, starting in late logarithmic growth, cells of the same age have a bi-modal distribution of oxidation status. A comparative proteomic analysis between these populations identified three key proteins, Hsp30, Dhh1, and Pnc1, which affect basal oxidation levels and may serve as first line of defense proteins in redox homeostasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.