Evidence map›Paper›PMID 29869062›Full record

ArticleMolecular imaging and biology2019

Tumor Formation of Adult Stem Cell Transplants in Rodent Arthritic Joints.

Fanny Chapelin, Aman Khurana, Mohammad Moneeb, Florette K Gray Hazard, Chun Fai Ray Chan, Hossein Nejadnik, Dita Gratzinger, Solomon Messing, Jason Erdmann, Amitabh Gaur and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular imaging and biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
0.7field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
  2. Article
  3. Stem cell therapy in pulmonary hypertension: current practice and future opportunities.European respiratory review : an official journal of the European Respiratory Society · 2023
    Review
  4. Stem Cell's Secretome Delivery Systems.Advanced pharmaceutical bulletin · 2023
    Review
  5. Article
  6. Article
  7. Review
  8. Blood Vessel Resident Human Stem Cells in Health and Disease.Stem cells translational medicine · 2022
    Review
  9. Article
  10. [Comparison of two methods for preparing knee osteochondral injury models in mice].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2021
    Article
  11. Review
  12. Perivascular Mesenchymal Progenitors for Bone Regeneration.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019
    Review
  13. Pericytes for Therapeutic Bone Repair.Advances in experimental medicine and biology
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Fanny ChapelinDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University, 725 Welch Rd, Rm 1665, Stanford, CA, 94305-5654, USA.
Aman KhuranaDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University, 725 Welch Rd, Rm 1665, Stanford, CA, 94305-5654, USA.
Mohammad MoneebDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University, 725 Welch Rd, Rm 1665, Stanford, CA, 94305-5654, USA.
Florette K Gray HazardDepartment of Pathology, Stanford University, Stanford, CA, USA.
Chun Fai Ray ChanBD biosciences, Custom Technology Team, La Jolla, CA, USA.
Hossein NejadnikDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University, 725 Welch Rd, Rm 1665, Stanford, CA, 94305-5654, USA.
Dita GratzingerDepartment of Pathology, Stanford University, Stanford, CA, USA.
Solomon MessingDepartment of Communication and Statistics, Stanford, CA, USA.
Jason ErdmannDepartment of Cytogenetics, Stanford University, Stanford, CA, USA.
Amitabh GaurBD biosciences, Custom Technology Team, La Jolla, CA, USA.
Heike E Daldrup-LinkDepartment of Radiology, Molecular Imaging Program at Stanford (MIPS), Stanford University, 725 Welch Rd, Rm 1665, Stanford, CA, 94305-5654, USA. H.E.Daldrup-Link@stanford.edu.
Stanford University · USBD Biosciences (United States) · USCAE Solutions (United States) · USStanford Medicine · US

Funding

Monitoring of Stem Cell Engraftment in Arthritic Joints with MR ImagingR01AR054458 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI DALDRUP-LINK, HEIKE ELIZABETH · 2008 to 2021
$6.7M
NIAMS NIH HHS R01 AR054458
6 · The paper itself

Abstract

purposeWhile imaging matrix-associated stem cell transplants aimed for cartilage repair in a rodent arthritis model, we noticed that some transplants formed locally destructive tumors. The purpose of this study was to determine the cause for this tumor formation in order to avoid this complication for future transplants. PROCEDURES: Adipose-derived stem cells (ADSC) isolated from subcutaneous adipose tissue were implanted into 24 osteochondral defects of the distal femur in ten athymic rats and two immunocompetent control rats. All transplants underwent serial magnetic resonance imaging (MRI) up to 6 weeks post-transplantation to monitor joint defect repair. Nine transplants showed an increasing size over time that caused local bone destruction (group 1), while 11 transplants in athymic rats (group 2) and 4 transplants in immunocompetent rats did not. We compared the ADSC implant size and growth rate on MR images, macroscopic features, histopathologic features, surface markers, and karyotypes of these presumed neoplastic transplants with non-neoplastic ADSC transplants.

resultsImplants in group 1 showed a significantly increased two-dimensional area at week 2 (p = 0.0092), 4 (p = 0.003), and 6 (p = 0.0205) compared to week 0, as determined by MRI. Histopathological correlations confirmed neoplastic features in group 1 with significantly increased size, cellularity, mitoses, and cytological atypia compared to group 2. Six transplants in group 1 were identified as malignant chondrosarcomas and three transplants as fibromyxoid sarcomas. Transplants in group 2 and immunocompetent controls exhibited normal cartilage features. Both groups showed a normal ADSC phenotype; however, neoplastic ADSC demonstrated a mixed population of diploid and tetraploid cells without genetic imbalance.

conclusionsADSC transplants can form tumors in vivo. Preventive actions to avoid in vivo tumor formations may include karyotyping of culture-expanded ADSC before transplantation. In addition, serial imaging of ADSC transplants in vivo may enable early detection of abnormally proliferating cell transplants.

Indexed as

Adult Stem CellsAnimalsArthritisBone NeoplasmsCells, CulturedCell Transformation, NeoplasticChondrosarcomaFemurFibromaJointsMagnetic Resonance ImagingMaleMesenchymal Stem CellsMesenchymal Stem Cell TransplantationRatsRats, NudeChondrosarcomasFibromyxoid sarcomasMagnetic resonance imagingMalignant tumors in vivoOsteochondral transplantsStem cell therapy

Identifiers

PMID29869062
PMCPMC6279627
OpenAlexW2805811035

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.