ArticlePlant cell reports2018
A novel VIGS method by agroinoculation of cotton seeds and application for elucidating functions of GhBI-1 in salt-stress response.
Article in Plant cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 66 citations in OpenAlex.
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- GhiPLATZ17 and GhiPLATZ22, zinc-dependent DNA-binding transcription factors, promote salt tolerance in upland cotton.Plant cell reports · 2024Article
- Identification and characterization of candidate genes for primary root length in Asiatic cotton (Gossypium arboreum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- A Cotyledon-based Virus-Induced Gene Silencing (Cotyledon-VIGS) approach to study specialized metabolism in medicinal plants.Plant methods · 2024Article
- Establishing a Virus-Induced Gene Silencing System inPlants (Basel, Switzerland) · 2023Article
- Principles and practice of virus induced gene silencing for functional genomics in plants.Virus genes · 2023Review
- Virus-Induced Gene Silencing (VIGS): A Powerful Tool for Crop Improvement and Its Advancement towards Epigenetics.International journal of molecular sciences · 2023Review
- Establishment of a Virus-Induced Gene-Silencing (VIGS) System in Tea Plant and Its Use in the Functional Analysis ofInternational journal of molecular sciences · 2022Article
- Screening and Verification of Photosynthesis and Chloroplast-Related Genes in Mulberry by Comparative RNA-Seq and Virus-Induced Gene Silencing.International journal of molecular sciences · 2022Article
- Plant biomacromolecule delivery methods in the 21st century.Frontiers in genome editing · 2022Review
- Identification of Raf-Like Kinases B Subfamily Genes inInternational journal of molecular sciences · 2021Article
- Host sunflower-induced silencing of parasitism-related genes confers resistance to invading Orobanche cumana.Plant physiology · 2021Article
- Molecular cloning and characterization of GhERF105, a gene contributing to the regulation of gland formation in upland cotton (Gossypium hirsutum L.).BMC plant biology · 2021Article
- A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants.Frontiers in plant science · 2021Review
- Comparative Phenotypic and Transcriptomic Analysis Reveals Key Responses of Upland Cotton to Salinity Stress During Postgermination.Frontiers in plant science · 2021Article
- Global analysis of expression profile of members of DnaJ gene families involved in capsaicinoids synthesis in pepper (Capsicum annuum L).BMC plant biology · 2020Article
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Authors and funding
9 authors at 4 institutions in 1 country.
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Abstract
key messageA VIGS method by agroinoculation of cotton seeds was developed for gene silencing in young seedlings and roots, and applied in functional analysis of GhBI-1 in response to salt stress. Virus-induced gene silencing (VIGS) has been widely used to investigate the functions of genes expressed in mature leaves, but not yet in young seedlings or roots of cotton (Gossypium hirsutum L.). Here, we developed a simple and effective VIGS method for silencing genes in young cotton seedlings and roots by soaking naked seeds in Agrobacterium cultures carrying tobacco rattle virus (TRV)-VIGS vectors. When the naked seeds were soaked in Agrobacterium cultures with an OD600 of 1.5 for 90 min, it was optimal for silencing genes effectively in young seedlings as clear photo-bleaching phenotype in the newly emerging leaves of pTRV:GhCLA1 seedlings were observed at 12-14 days post inoculation. Silencing of GhPGF (cotton pigment gland formation) by this method resulted in a 90% decrease in transcript abundances of the gene in roots at the early development stage. We further used the tool to investigate function of GhBI-1 (cotton Bax inhibitor-1) gene in response to salt stress and demonstrated that GhBI-1 might play a protective role under salt stress by suppressing stress-induced cell death in cotton. Our results showed that the newly established VIGS method is a powerful tool for elucidating functions of genes in cotton, especially the genes expressed in young seedlings and roots.
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