ArticleClinical infectious diseases : an official publication of the Infectious Diseases Society of America2019
Etiology of Sepsis in Uganda Using a Quantitative Polymerase Chain Reaction-based TaqMan Array Card.
Article in Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04618198 (A Randomized Clinical Trial of Early Empiric Anti-Mycobacterium Tuberculosis Therapy for Sepsis in Sub-Saharan Africa), which is not on this map. Cited by 45 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
A Randomized Clinical Trial of Early Empiric Anti-Mycobacterium Tuberculosis Therapy for Sepsis in Sub-Saharan Africa
Who cites it
45 citing papers in PubMed, 3 syntheses or guidelines pooled it, 68 citations in OpenAlex.
- Etiologies of community-acquired febrile illness identified by TaqMan Array Card qPCR on blood samples: a systematic review and meta-analysis.Journal of clinical microbiology · 2026Pooled it
- Clinical Utility of Molecular Tests for Guiding Therapeutic Decisions in Bloodstream Staphylococcal Infections: A Meta-Analysis.Frontiers in pediatrics · 2021Pooled it
- Aetiology and outcomes of sepsis in adults in sub-Saharan Africa: a systematic review and meta-analysis.Critical care (London, England) · 2019Pooled it
- Immediate or high-dose antituberculosis therapy for HIV-related sepsis in Tanzania and Uganda (ATLAS): a phase 3, open-label, randomised, controlled, 2 × 2 factorial, superiority trial.The Lancet. Infectious diseases · 2026Trial
- Etiology of severe invasive infections in young infants in rural settings in sub-Saharan Africa.PloS one · 2022Trial
- Measles Without Rash During Acute Febrile Illness Surveillance in Tanzania, 2023-2024.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2026Article
- C-reactive Protein in TB Meningitis: Signal, Surrogate, or Saboteur?Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2026Article
- Aetiology of sepsis in adults living with HIV in East Africa: a secondary analysis of an open-label, multicentre, randomised, controlled phase 3 trial.EClinicalMedicine · 2026Article
- Characteristics and outcomes of adult emergency ward patients with sepsis who received corticosteroids at the Mbarara Regional Referral Hospital in Uganda.Emergency medicine journal : EMJ · 2025Observational
- Bacteriological profile and antimicrobial resistance in sepsis cases in intensive care units in Lubumbashi: challenges and perspectives.Annals of clinical microbiology and antimicrobials · 2025Article
- Deploying Metagenomics to Characterize Microbial Pathogens During Outbreak of Acute Febrile Illness Among Children in Tanzania.Pathogens (Basel, Switzerland) · 2025Article
- High throughput screening for human disease associated-pathogens and antimicrobial resistance genes in migratory birds at ten habitat sites in China.BMC microbiology · 2025Article
- Review
- Detection of pathogens associated with acute febrile illness in children under five years of age in rural Tanzania.Scientific reports · 2025Article
- TaqMan Array Card real-time polymerase chain reaction panel to detect pathogens in whole blood of febrile inpatients in northern Tanzania, 2016-2019.Tropical medicine & international health : TM & IH · 2025Article
- Automated post-run analysis of arrayed quantitative PCR amplification curves using machine learning.Gates open research · 2025Article
- Multiplexed bacterial pathogen detection and clinical characteristics of orthopedic infection in hospitalized patients.Frontiers in cellular and infection microbiology · 2024Article
- The Universal Vital Assessment (UVA) score at 6 hours post-resuscitation predicts mortality in hospitalized adults with severe sepsis in Mbarara, Uganda.PLOS global public health · 2024Article
- Cryptococcal meningitis.Nature reviews. Disease primers · 2023Review
- Etiology of Fever and Associated Outcomes Among Adults Receiving Chemotherapy for the Treatment of Solid Tumors in Uganda.Open forum infectious diseases · 2023Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Background: Knowledge of causes of sepsis in sub-Saharan Africa is limited. A better understanding of the microbiology of bloodstream infections could improve outcomes. Methods: We used a quantitative polymerase chain reaction (qPCR)-based TaqMan Array Card (TAC) to directly test for 43 targets from whole blood. We analyzed 336 cryopreserved specimens from adult Ugandans with sepsis enrolled in a multisite study; 84% were infected with human immunodeficiency virus. We compared qPCR TAC results with blood culture and determined the association of qPCR with study participant outcomes using logistic regression. Results: The most frequently detected targets were cytomegalovirus (CMV, n = 139, 41%), Mycobacterium tuberculosis (TB, n = 70, 21%), Plasmodium (n = 35, 10%), and Streptococcus pneumoniae (n = 31, 9%). Diagnostic performance varied by target with qPCR sensitivity averaging 61 ± 28% and specificity 98 ± 3% versus culture. In multivariable analysis, independent factors associated with in-hospital mortality included CMV viremia (adjusted odds ratio [aOR] 3.2, 95% confidence interval [CI], 1.8-5.5; p < .01) and TB qPCR-positivity, whether blood culture-positive (aOR 4.6, 95% CI, 2.1-10.0; p < .01) or blood culture-negative (aOR 2.9, 95% CI, 1.2-6.9; p = .02). Conclusions: Using qPCR TAC on direct blood specimens, CMV and TB were the most commonly identified targets and were independently associated with increased in-hospital mortality. qPCR TAC screening of blood for multiple targets may be useful to guide triage and treatment of sepsis in sub-Saharan Africa.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.