Evidence map›Paper›PMID 29867859›Full record

ArticleFrontiers in microbiology2018

Development of Colloidal Gold-Based Immunochromatographic Assay for Rapid Detection of Goose Parvovirus.

Xianglong Yu, Lei Wei, Hao Chen, Xiaoyu Niu, Yanguo Dou, Jing Yang, Zhenzhong Wang, Yi Tang, Youxiang Diao

Open access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 31 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Xianglong YuCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Lei WeiTai'an City Central Hospital, Tai'an, China.
Hao ChenCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Xiaoyu NiuCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Yanguo DouCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Jing YangCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Zhenzhong WangCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Yi TangCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Youxiang DiaoCollege of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Shandong Agricultural University · CNTaian City Central Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Goose parvovirus (GPV) remains as a worldwide problem in goose industry. For this reason, it is necessary to develop a new diagnostic approach that is easier and faster than conventional tests. A rapid immunochromatographic assay based on antibody colloidal gold nanoparticles specific to GPV was developed for the detection of GPV in goose allantoic fluid and supernatant of tissue homogenate. The monoclonal antibodies (Mab) was produced by immunizing the BALB/c mice with purified GPV suspension, and the polyclonal antibody (pAb) was produced by immunizing the rabbits with recombinant VP3 protein. The colloidal gold was prepared by the reduction of gold salt with sodium citrate coupled with Mab against GPV. The optimal concentrations of the coating antibody and capture antibody were determined to be 1.6 mg/ml and 9 μg/ml. With visual observation, the lower limit was found to be around 1.2 μg/ml. Common diseases of goose were tested to evaluate the specificity of the immune colloidal gold (ICG) strip, and no cross-reaction was observed. The clinical detection was examined by carrying out the ICG strip test with 92 samples and comparing the results of these tests with those obtained via agar diffusion test and polymerase chain reaction (PCR) test. Therefore, the ICG strip test was a sufficiently sensitive and accurate detection method for a rapid screening of GPV.

Indexed as

colloidal goldgoose parvovirusimmunochromatographic stripmonoclonal antibodyrapid detection

Identifiers

PMID29867859
PMCPMC5961387
OpenAlexW2802513291

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.