ArticleFrontiers in microbiology2018
Development of a Custom MALDI-TOF MS Database for Species-Level Identification of Bacterial Isolates Collected From Spacecraft and Associated Surfaces.
Article in Frontiers in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- IDBac: an open-access web platform to identify bacteria and analyze relationships in culture collections using MALDI-TOF mass spectrometry.Nature communications · 2026Article
- IDBac: an open-access web platform and compendium for the identification of bacteria by MALDI-TOF mass spectrometry.bioRxiv : the preprint server for biology · 2025Article
- Identification and Differentiation of Non-HemolyticMolecules (Basel, Switzerland) · 2025Article
- Spectral Precision: Recent Advances in Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Pathogen Detection and Resistance Profiling.Microorganisms · 2025Review
- Use of MALDI-TOF mass spectrometry and IDBac to mine for understudied bacterial genera from the environment.ISME communications · 2025Article
- Resolution of MALDI-TOF compared to whole genome sequencing for identification ofFrontiers in microbiology · 2025Article
- Isolation, Selection, and Identification of Keratinolytic Bacteria for Green Management of Keratin Waste.Molecules (Basel, Switzerland) · 2024Article
- Comparison of MALDI-TOF mass spectrometry and 16S rDNA sequencing for identification of environmental bacteria: a case study of cave mussel-associated culturable microorganisms.Environmental science and pollution research international · 2024Article
- Insights into population adaptation and biodiversity of lactic acid bacteria in challenged date palm leaves silaging, using MALDI-TOF MS.Current research in microbial sciences · 2024Article
- A comprehensive technology strategy for microbial identification and contamination investigation in the sterile drug manufacturing facility-a case study.Frontiers in microbiology · 2024Article
- Study of diversity of mineral-forming bacteria in sabkha mats and sediments of mangrove forest in Qatar.Biotechnology reports (Amsterdam, Netherlands) · 2023Article
- Draft Genome Sequences of Spacecraft-Associated Microbes Isolated from Six NASA Missions.Microbiology resource announcements · 2023Article
- A MALDI-TOF MS library for rapid identification of human commensal gut bacteria from the classFrontiers in microbiology · 2023Article
- Relationship between bacterial phylotype and specialized metabolite production in the culturable microbiome of two freshwater sponges.ISME communications · 2022Article
- MALDI-TOF MS Using a Custom-Made Database, Biomarker Assignment, or Mathematical Classifiers Does Not DifferentiateMicroorganisms · 2022Article
- Current Scenario and Challenges in the Direct Identification of Microorganisms Using MALDI TOF MS.Microorganisms · 2021Review
- Evaluation by MALDI-TOF MS and PCA of the diversity of biosurfactants and their producing bacteria, as adaption to weathered oil components.Biotechnology reports (Amsterdam, Netherlands) · 2021Article
- A new approach to identifying pathogens, with particular regard to viruses, based on capillary electrophoresis and other analytical techniques.Trends in analytical chemistry : TRAC · 2021Review
- Approaches for characterizing and tracking hospital-associated multidrug-resistant bacteria.Cellular and molecular life sciences : CMLS · 2021Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since the 1970s, the Planetary Protection Group at the Jet Propulsion Laboratory (JPL) has maintained an archive of spacecraft-associated bacterial isolates. Identification of these isolates was routinely performed by sequencing the 16S rRNA gene. Although this technique is an industry standard, it is time consuming and has poor resolving power for some closely related taxa. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry is widely used in clinical diagnostics and is a promising method to substitute standard 16S rRNA sequencing. However, manufacturer-provided databases lack the bacterial diversity found in spacecraft-assembly cleanrooms. This study reports the development of the first custom database of MALDI-TOF MS profiles of bacterial isolates obtained from spacecraft and associated cleanroom environments. With the use of this in-house database, 454 bacterial isolates were successfully identified in concurrence with their 16S rRNA sequence-based classifications. Additionally, MALDI-TOF MS resolved strain-level variations, identified potential novel species and distinguished between members of taxonomic groups, which is not possible using conventional 16S rRNA sequencing. MALDI-TOF MS has proved to be an accurate, high-throughput approach for real-time identification of bacterial isolates during the spacecraft assembly process.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.