ArticleScientific reports2018
High sample throughput genotyping for estimating C-lineage introgression in the dark honeybee: an accurate and cost-effective SNP-based tool.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Modelling the impacts of imports and non-native subspecies hybridisation in honeybees.Heredity · 2026Article
- Population Genetic Study ofEcology and evolution · 2026Article
- Country specific hybridization of honey bees from lineage M.GigaScience · 2026Article
- Assessing structure-function impacts on Vitellogenin by leveraging allelic variant found in honey bee subspeciesiScience · 2025Article
- Accurate Detection ofEcology and evolution · 2024Article
- HBeeID: a molecular tool that identifies honey bee subspecies from different geographic populations.BMC bioinformatics · 2024Article
- Estimates of the vitality and performances ofPeerJ · 2024Article
- When One's Not Enough: Colony Pool-Seq Outperforms Individual-Based Methods for Assessing Introgression inInsects · 2023Article
- Honey bee (Apis mellifera) wing images: a tool for identification and conservation.GigaScience · 2023Article
- Using the Software DeepWings© to Classify Honey Bees across Europe through Wing Geometric Morphometrics.Insects · 2022Article
- A genotyping by sequencing approach can disclose Apis mellifera population genomic information contained in honey environmental DNA.Scientific reports · 2022Article
- Complex population structure and haplotype patterns in the Western European honey bee from sequencing a large panel of haploid drones.Molecular ecology resources · 2022Article
- Identification of 121 variants of honey bee Vitellogenin protein sequences with structural differences at functional sites.Protein science : a publication of the Protein Society · 2022Article
- Structure prediction of honey bee vitellogenin: a multi-domain protein important for insect immunity.FEBS open bio · 2022Article
- Honey bee populations of the USA display restrictions in their mtDNA haplotype diversity.Frontiers in genetics · 2022Article
- Thrice out of Asia and the adaptive radiation of the western honey bee.Science advances · 2021Article
- A SNP assay for assessing diversity in immune genes in the honey bee (Apis mellifera L.).Scientific reports · 2021Article
- Distribution of the MainInsects · 2021Article
- Authoritative subspecies diagnosis tool for European honey bees based on ancestry informative SNPs.BMC genomics · 2021Article
- Tool for genomic selection and breeding to evolutionary adaptation: Development of a 100K single nucleotide polymorphism array for the honey bee.Ecology and evolution · 2020Article
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Authors and funding
12 authors.
Funding
Abstract
The natural distribution of the honeybee (Apis mellifera L.) has been changed by humans in recent decades to such an extent that the formerly widest-spread European subspecies, Apis mellifera mellifera, is threatened by extinction through introgression from highly divergent commercial strains in large tracts of its range. Conservation efforts for A. m. mellifera are underway in multiple European countries requiring reliable and cost-efficient molecular tools to identify purebred colonies. Here, we developed four ancestry-informative SNP assays for high sample throughput genotyping using the iPLEX Mass Array system. Our customized assays were tested on DNA from individual and pooled, haploid and diploid honeybee samples extracted from different tissues using a diverse range of protocols. The assays had a high genotyping success rate and yielded accurate genotypes. Performance assessed against whole-genome data showed that individual assays behaved well, although the most accurate introgression estimates were obtained for the four assays combined (117 SNPs). The best compromise between accuracy and genotyping costs was achieved when combining two assays (62 SNPs). We provide a ready-to-use cost-effective tool for accurate molecular identification and estimation of introgression levels to more effectively monitor and manage A. m. mellifera conservatories.
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