ArticleScientific reports2018
New universal ITS2 primers for high-resolution herbivory analyses using DNA metabarcoding in both tropical and temperate zones.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed.
- Accounting for Variability Across Years, Depths, and Non-Target Reads Improves Quantification of Root Community Composition in Plant Mixtures.Molecular ecology resources · 2026Article
- Using next-generation sequencing to unravel the diet composition of Burrowing Parrots over the annual cycle.Die Naturwissenschaften · 2026Article
- DNA metabarcoding reveals dietary niche partitioning among threatened Himalayan Ungulates based on chloroplast rbcL and nuclear ITS2 markers.BMC ecology and evolution · 2026Article
- Molecular Genetic Characterization of the Diet of Limestone and Rainforest Langurs.Ecology and evolution · 2026Article
- DIY: A Practical Field-to-Sequencer Workflow for Metabarcoding the Diet of Terrestrial Carnivore Species.Molecular ecology resources · 2026Article
- Seasonal Dynamics of the Gut Microbiome in Urban Feral Pigeons Are Associated With Environmental Conditions, Not With Diet Shifts.Ecology and evolution · 2026Article
- Evaluating the Quantitative Accuracy and Application of DNA Metabarcoding for Dietary Reconstruction in Ruminants.Ecology and evolution · 2026Article
- Mock Plant Communities and a Large Mammal Case Study Reveal ITS2 Primer Bias Against Graminoids.Ecology and evolution · 2025Article
- Threatened endemic arthropods and vertebrates partition their diets with non-native ants in an isolated island ecosystem.Ecology · 2025Article
- Spatio-Temporal Variation in Diet Among Age and Sex Cohorts of a Model Generalist Bird Species, the Great TitEcology and evolution · 2025Article
- Authentication of the Botanical Origin of Honey:Journal of agricultural and food chemistry · 2025Article
- Comparative Analysis of Pasture Composition: DNA Metabarcoding Versus Quadrat-Based Botanical Surveys in Experimental Grassland Plots.Ecology and evolution · 2025Article
- Article
- Pot-pollen DNA barcoding as a tool to determine the diversity of plant species visited by Ecuadorian stingless bees.PloS one · 2025Article
- The InBIO Barcoding Initiative Database: contribution to the knowledge of DNA barcodes of the vascular plants of north-eastern Portugal.Biodiversity data journal · 2025Article
- High Resource Overlap and a Consistently Generalised Pattern of Interactions in a Bat-Flower Network in a Seasonally Dry Landscape.Ecology and evolution · 2024Article
- DNA metabarcoding analysis of the bare-nosed wombat (Ecology and evolution · 2024Article
- Metabarcoding analysis provides insight into the link between prey and plant intake in a large alpine cat carnivore, the snow leopard.Royal Society open science · 2024Article
- Semi-automated sequence curation for reliable reference datasets in ITS2 vascular plant DNA (meta-)barcoding.Scientific data · 2024Article
- Consumer identity but not food availability affects carabid diet in cereal crops.Journal of pest science · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA metabarcoding is a rapidly growing technique for obtaining detailed dietary information. Current metabarcoding methods for herbivory, using a single locus, can lack taxonomic resolution for some applications. We present novel primers for the second internal transcribed spacer of nuclear ribosomal DNA (ITS2) designed for dietary studies in Mauritius and the UK, which have the potential to give unrivalled taxonomic coverage and resolution from a short-amplicon barcode. In silico testing used three databases of plant ITS2 sequences from UK and Mauritian floras (native and introduced) totalling 6561 sequences from 1790 species across 174 families. Our primers were well-matched in silico to 88% of species, providing taxonomic resolution of 86.1%, 99.4% and 99.9% at the species, genus and family levels, respectively. In vitro, the primers amplified 99% of Mauritian (n = 169) and 100% of UK (n = 33) species, and co-amplified multiple plant species from degraded faecal DNA from reptiles and birds in two case studies. For the ITS2 region, we advocate taxonomic assignment based on best sequence match instead of a clustering approach. With short amplicons of 187-387 bp, these primers are suitable for metabarcoding plant DNA from faecal samples, across a broad geographic range, whilst delivering unparalleled taxonomic resolution.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.