ArticleProceedings of the National Academy of Sciences of the United States of America2018
Evolutionary convergence in lignin-degrading enzymes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 79 citations in OpenAlex.
- Increased temperature enhances microbial-mediated lignin decomposition in river sediment.Microbiome · 2025Article
- Exoproteomic Study and Transcriptional Responses of Laccase and Ligninolytic Peroxidase Genes of White-Rot FungusInternational journal of molecular sciences · 2023Article
- Adaptations of Pseudoxylaria towards a comb-associated lifestyle in fungus-farming termite colonies.The ISME journal · 2023Article
- Tree mycorrhizal association types control biodiversity-productivity relationship in a subtropical forest.Science advances · 2023Article
- Evaluation of the lignocellulose degradation potential of Mediterranean forests soil microbial communities through diversity and targeted functional metagenomics.Frontiers in microbiology · 2023Article
- Comparative analysis of simulatedFrontiers in microbiology · 2023Article
- Tandem metalloenzymes gate plant cell entry by pathogenic fungi.Science advances · 2022Article
- Designed High-Redox Potential Laccases Exhibit High Functional Diversity.ACS catalysis · 2022Article
- Fungal dye-decolorizing peroxidase diversity: roles in either intra- or extracellular processes.Applied microbiology and biotechnology · 2022Article
- Stable and Functionally Diverse Versatile Peroxidases Designed Directly from Sequences.Journal of the American Chemical Society · 2022Article
- Using the Evolutionary History of Proteins to Engineer Insertion-Deletion Mutants from Robust, Ancestral Templates Using Graphical Representation of Ancestral Sequence Predictions (GRASP).Methods in molecular biology (Clifton, N.J.) · 2022Article
- Ancestral sequence reconstruction as a tool to study the evolution of wood decaying fungi.Frontiers in fungal biology · 2022Review
- Metagenomics analysis of the effects ofPeerJ · 2022Article
- Gene family expansions and transcriptome signatures uncover fungal adaptations to wood decay.Environmental microbiology · 2021Article
- Agaricales Mushroom Lignin Peroxidase: From Structure-Function to Degradative Capabilities.Antioxidants (Basel, Switzerland) · 2021Article
- A plant host, Nicotiana benthamiana, enables the production and study of fungal lignin-degrading enzymes.Communications biology · 2021Article
- Setting the stage for evolution of a new enzyme.Current opinion in structural biology · 2021Review
- Textile Dye Biodecolorization by Manganese Peroxidase: A Review.Molecules (Basel, Switzerland) · 2021Review
- The Termite Fungal CultivarmBio · 2021Article
- Mutational and structural analysis of an ancestral fungal dye-decolorizing peroxidase.The FEBS journal · 2021Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The resurrection of ancestral enzymes of now-extinct organisms (paleogenetics) is a developing field that allows the study of evolutionary hypotheses otherwise impossible to be tested. In the present study, we target fungal peroxidases that play a key role in lignin degradation, an essential process in the carbon cycle and often a limiting step in biobased industries. Ligninolytic peroxidases are secreted by wood-rotting fungi, the origin of which was recently established in the Carboniferous period associated with the appearance of these enzymes. These first peroxidases were not able to degrade lignin directly and used diffusible metal cations to attack its phenolic moiety. The phylogenetic analysis of the peroxidases of Polyporales, the order in which most extant wood-rotting fungi are included, suggests that later in evolution these enzymes would have acquired the ability to degrade nonphenolic lignin using a tryptophanyl radical interacting with the bulky polymer at the surface of the enzyme. Here, we track this powerful strategy for lignin degradation as a phenotypic trait in fungi and show that it is not an isolated event in the evolution of Polyporales. Using ancestral enzyme resurrection, we study the molecular changes that led to the appearance of the same surface oxidation site in two distant peroxidase lineages. By characterization of the resurrected enzymes, we demonstrate convergent evolution at the amino acid level during the evolution of these fungi and track the different changes leading to phylogenetically distant ligninolytic peroxidases from ancestors lacking the ability to degrade nonphenolic lignin.
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