ArticleAnalytica chimica acta2018
Electrokinetic transport properties of deoxynucleotide monophosphates (dNMPs) through thermoplastic nanochannels.
Article in Analytica chimica acta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- The evolution of nanopore measurements: from biological out-of-plane pores to plastic in-plane pores.Lab on a chip · 2026Review
- Millisecond Label-Free Single Peptide Detection and Identification Using Nanoscale Electrochromatography and Resistive Pulse Sensing.Analytical chemistry · 2025Article
- Novel thermoplastic microvalves based on an elastomeric cyclic olefin copolymer.Lab on a chip · 2024Article
- Detection and identification of single ribonucleotide monophosphates using a dual in-plane nanopore sensor made in a thermoplasticLab on a chip · 2024Article
- Nano-injection molding with resin mold inserts for prototyping of nanofluidic devices for single molecular detection.Lab on a chip · 2023Article
- In-plane Extended Nano-coulter Counter (XnCC) for the Label-free Electrical Detection of Biological Particles.Electroanalysis · 2022Article
- Nanofluidic devices for the separation of biomolecules.Journal of chromatography. A · 2022Review
- Label-Free Identification of Single Mononucleotides by Nanoscale Electrophoresis.Small (Weinheim an der Bergstrasse, Germany) · 2021Article
- DNA translocation through pH-dependent soft nanopores.European biophysics journal : EBJ · 2021Article
- Tailoring Thermoplastic In-Plane Nanopore Size by Thermal Fusion Bonding for the Analysis of Single Molecules.ACS sensors · 2021Article
- Thermoplastic nanofluidic devices for identifying abasic sites in single DNA molecules.Lab on a chip · 2021Article
- Solid-phase XRN1 reactions for RNA cleavage: application in single-molecule sequencing.Nucleic acids research · 2021Article
- Electrokinetic identification of ribonucleotide monophosphates (rNMPs) using thermoplastic nanochannels.Journal of chromatography. A · 2021Article
- Open-tubular nanoelectrochromatography (OT-NEC): gel-free separation of single stranded DNAs (ssDNAs) in thermoplastic nanochannels.Electrophoresis · 2020Article
- Cell Separations and Sorting.Analytical chemistry · 2020Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The electrokinetic behavior of molecules in nanochannels (<100 nm in length) have generated interest due to the unique transport properties observed that are not seen in microscale channels. These nanoscale dependent transport properties include transverse electromigration arising from partial electrical double layer overlap, enhanced solute/wall interactions due to the small channel diameter, and field-dependent intermittent motion produced by surface roughness. In this study, the electrokinetic transport properties of deoxynucleotide monophosphates (dNMPs) were investigated, including the effects of electric field strength, surface effects, and composition of the carrier electrolyte (ionic concentration and pH). The dNMPs were labeled with a fluorescent reporter (ATTO 532) to allow tracking of the electrokinetic transport of the dNMPs through a thermoplastic nanochannel fabricated via nanoimprinting (110 nm × 110 nm, width × depth, and 100 μm in length). We discovered that the transport properties in plastic nanochannels of the dye-labeled dNMPs produced differences in their apparent mobilities that were not seen using microscale columns. We built histograms for each dNMP from their apparent mobilities under different operating conditions and fit the histograms to Gaussian functions from which the separation resolution could be deduced as a metric to gage the ability to identify the molecule based on their apparent mobility. We found that the resolution ranged from 0.73 to 2.13 at pH = 8.3. Changing the carrier electrolyte pH > 10 significantly improved separation resolution (0.80-4.84) and reduced the standard deviation in the Gaussian fit to the apparent mobilities. At low buffer concentrations, decreases in separation resolution and increased standard deviations in Gaussian fits to the apparent mobilities of dNMPs were observed due to the increased thickness of the electric double layer leading to a partial parabolic flow profile. The results secured for the dNMPs in thermoplastic nanochannels revealed a high identification efficiency (>99%) in most cases for the dNMPs due to differences in their apparent mobilities when using nanochannels, which could not be achieved using microscale columns.
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Registered trials
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