ArticleAnalytica chimica acta2018
SPME-GC×GC-TOF MS fingerprint of virally-infected cell culture: Sample preparation optimization and data processing evaluation.
Article in Analytica chimica acta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Evaluation of peak alignment performance of commercial and open-source tools for GC×GC-MS based non-targeted screening.Briefings in bioinformatics · 2026Article
- Exploratory Volatilome Profiling of Inflammation in Skin Fibroblasts: A Proof-of-Concept Study.International journal of molecular sciences · 2026Article
- A large scale study of portable sweat test sensor for accurate, non-invasive and rapid COVID-19 screening based on volatile compound marker detection.Scientific reports · 2024Article
- The isolation strategy and chemical analysis of oil cells from Asari Radix et Rhizoma.Plant methods · 2024Article
- Headspace Solid-Phase Micro-Extraction Method Optimization and Evaluation for the Volatile Compound Extraction of Bronchoalveolar Lung Lavage Fluid Samples.Separations · 2024Article
- Article
- Fisher ratio feature selection by manual peak area calculations on comprehensive two-dimensional gas chromatography data using standard mixtures with variable composition, storage, and interferences.Analytical and bioanalytical chemistry · 2023Article
- Exhaled breath biomarkers of influenza infection and influenza vaccination.Journal of breath research · 2021Article
- Investigating Bacterial Volatilome for the Classification and Identification of Mycobacterial Species by HS-SPME-GC-MS and Machine Learning.Molecules (Basel, Switzerland) · 2021Article
- Automating and Extending Comprehensive Two-Dimensional Gas Chromatography Data Processing by Interfacing Open-Source and Commercial Software.Analytical chemistry · 2020Article
- Multimodal combination of GC × GC-HRTOFMS and SIFT-MS for asthma phenotyping using exhaled breath.Scientific reports · 2020Article
- Advances in the application of comprehensive two-dimensional gas chromatography in metabolomics.Trends in analytical chemistry : TRAC · 2018Article
- Exhaled human breath analysis in active pulmonary tuberculosis diagnostics by comprehensive gas chromatography-mass spectrometry and chemometric techniques.Journal of breath research · 2018Article
- VOC breath profile in spontaneously breathing awake swine during Influenza A infection.Scientific reports · 2018Article
Corrections and comments
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7 authors.
Funding
Abstract
Untargeted metabolomics study of volatile organic compounds produced by different cell cultures is a field that has gained increasing attention over the years. Solid-phase microextraction has been the sampling technique of choice for most of the applications mainly due to its simplicity to implement. However, a careful optimization of the analytical conditions is necessary to obtain the best performances, which are highly matrix-dependent. In this work, five different solid-phase microextraction fibers were compared for the analysis of the volatiles produced by cell culture infected with the human respiratory syncytial virus. A central composite design was applied to determine the best time-temperature combination to maximize the extraction efficiency and the salting-out effect was evaluated as well. The linearity of the optimized method, along with limits of detection and quantification and repeatability was assessed. Finally, the effect of i) different normalization techniques (i.e. z-score and probabilistic quotient normalization), ii) data transformation (i.e. in logarithmic scale), and iii) different feature selection algorithms (i.e. Fisher ratio and random forest) on the capability of discriminating between infected and not-infected cell culture was evaluated.
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