ArticleMolecular brain2018
Excitation wavelength optimization improves photostability of ASAP-family GEVIs.
Article in Molecular brain, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Sub-millivolt voltage imaging reveals gap junction-mediated bioelectric contact inhibition.Nature communications · 2026Article
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- Absolute Membrane Potential Recording with ASAP-Type Genetically Encoded Voltage Indicators Using Fluorescence Lifetime Imaging.ACS chemical neuroscience · 2025Article
- Obtaining artifact-corrected signals in fiber photometry via isosbestic signals, robust regression, and dF/F calculations.Neurophotonics · 2025Article
- Ultrafast optical imaging techniques for exploring rapid neuronal dynamics.Neurophotonics · 2025Review
- An Ultrasensitive Genetically Encoded Voltage Indicator Uncovers the Electrical Activity of Non-Excitable Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Photoswitching alters fluorescence readout of jGCaMP8 CaProceedings of the National Academy of Sciences of the United States of America · 2023Article
- A positively tuned voltage indicator for extended electrical recordings in the brain.Nature methods · 2023Article
- High-speed low-light in vivo two-photon voltage imaging of large neuronal populations.Nature methods · 2023Article
- Monitoring of compound resting membrane potentials of cell cultures with ratiometric genetically encoded voltage indicators.Communications biology · 2021Article
- PatcherBot: a single-cell electrophysiology robot for adherent cells and brain slices.Journal of neural engineering · 2019Article
- Single-Neuron Level One-Photon Voltage Imaging With Sparsely Targeted Genetically Encoded Voltage Indicators.Frontiers in cellular neuroscience · 2019Article
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Authors and funding
5 authors.
Funding
Abstract
Recent interest in high-throughput recording of neuronal activity has motivated rapid improvements in genetically encoded calcium or voltage indicators (GECIs or GEVIs) for all-optical electrophysiology. Among these probes, the ASAPs, a series of voltage indicators based on a variant of circularly permuted green fluorescent protein (cpGFP) and a conjugated voltage sensitive domain (VSD), are capable of detecting both action potentials and subthreshold neuronal activities. Here we show that the ASAPs, when excited by blue light, undergo reversible photobleaching. We find that this fluorescence loss induced by excitation with 470-nm light can be substantially reversed by low-intensity 405-nm light. We demonstrate that 405-nm and 470-nm co-illumination significantly improved brightness and thereby signal-to-noise ratios during voltage imaging compared to 470-nm illumination alone. Illumination with a single wavelength of 440-nm light also produced similar improvements. We hypothesize that reversible photobleaching is related to cis-trans isomerization and protonation of the GFP chromophore of ASAP proteins. Amino acids that influence chromophore isomerization are potential targets of point mutations for future improvements.
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