ArticleBMC genomics2018
Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fish.
Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 32 citations in OpenAlex.
- Differential expression of reproduction and sex-determining genes in the gonads of genotypic and temperature-induced males of gibel carp (Carassius gibelio).Scientific reports · 2025Article
- Comparative genome anatomy reveals evolutionary insights into a unique amphitriploid fish.Nature ecology & evolution · 2022Article
- Changes in Ploidy Drive Reproduction Transition and Genomic Diversity in a Polyploid Fish Complex.Molecular biology and evolution · 2022Article
- Sex determination mechanisms and sex control approaches in aquaculture animals.Science China. Life sciences · 2022Review
- Two duplicated gsdf homeologs cooperatively regulate male differentiation by inhibiting cyp19a1a transcription in a hexaploid fish.PLoS genetics · 2022Article
- Genomic anatomy of male-specific microchromosomes in a gynogenetic fish.PLoS genetics · 2021Article
- Comparative transcriptomic analysis reveals an association of gibel carp fatty liver with ferroptosis pathway.BMC genomics · 2021Article
- Functional Divergence of Multiple Duplicated Foxl2 Homeologs and Alleles in a Recurrent Polyploid Fish.Molecular biology and evolution · 2021Article
- Genotypic Males Play an Important Role in the Creation of Genetic Diversity in Gynogenetic Gibel Carp.Frontiers in genetics · 2021Article
- Whole Genome Incorporation and Epigenetic Stability in a Newly Synthetic Allopolyploid of Gynogenetic Gibel Carp.Genome biology and evolution · 2018Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundCoexistence and transition of diverse sex determination strategies have been revealed in some ectothermic species, but the variation between males caused by different sex determination strategies and the underlying mechanism remain unclear. Here, we used the gynogenetic gibel carp (Carassius gibelio) with both genotypic sex determination (GSD) and temperature-dependent sex determination (TSD) strategies to illustrate this issue.
resultsWe found out that males of GSD and TSD in gibel carp had similar morphology, testicular histology, sperm structure and sperm vitality. However, when maternal individuals were mated with males of GSD, sperm nucleus swelling and fusing with the female pronucleus were observed in the fertilized eggs. On the contrary, when maternal individuals were mated with males of TSD, sperm nucleus remained in the condensed status throughout the whole process. Subsequently, semen proteomics analysis unveiled that DNA replication and gene expression-related pathways were inhibited in the sperm from males of TSD compared to males of GSD, and most differentially expressed proteins associated with DNA replication, transcription and translation were down-regulated. Moreover, via BrdU incorporation and immunofluorescence detection, male nucleus replication was revealed to be present in the fertilized eggs by the sperm from males of GSD, but absent in the fertilized eggs by the sperm from males of TSD.
conclusionsThese findings indicate that DNA replication and gene expression-related pathways are associated with the distinct sperm nucleus development behaviors in fertilized eggs in response to the sperm from males of GSD and TSD. And this study is the first attempt to screen the differences between males determined via GSD and TSD in gynogenetic species, which might give a hint for understanding evolutionary adaption of diverse sex determination mechanisms in unisexual vertebrates.
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