Evidence map›Paper›PMID 29864529›Full record

ArticleJournal of structural biology2018

cryoem-cloud-tools: A software platform to deploy and manage cryo-EM jobs in the cloud.

Michael A Cianfrocco, Indrajit Lahiri, Frank DiMaio, Andres E Leschziner

Abstract read
In one paragraph

Article in Journal of structural biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 21 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Review
  6. Detection of Coronaviruses Using RNA Toehold Switch Sensors.International journal of molecular sciences · 2021
    Article
  7. Article
  8. Article
  9. Review
  10. Article
  11. Article
  12. Methods for merging data sets in electron cryo-microscopy.Acta crystallographica. Section D, Structural biology · 2019
    Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Michael A CianfroccoDepartment of Cellular & Molecular Medicine, University of California - San Diego, La Jolla, CA, United States; Life Sciences Institute, Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, United States. Electronic address: mcianfro@umich.edu.
Indrajit LahiriDepartment of Cellular & Molecular Medicine, University of California - San Diego, La Jolla, CA, United States.
Frank DiMaioDepartment of Biochemistry, University of Washington, Seattle, United States; Institute for Protein Design, University of Washington, Seattle, WA, United States.
Andres E LeschzinerDepartment of Cellular & Molecular Medicine, University of California - San Diego, La Jolla, CA, United States; Section of Molecular Biology, Division of Biology, University of California - San Diego, La Jolla, CA, United States.
University of California, San Diego · USUniversity of Washington · US

Funding

Regulation of Cytoplasmic DyneinR01GM107214 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI LESCHZINER, ANDRES · 2014 to 2021
$3.1M
A comparative structural study of ATP-dependent chromatin remodeling complexesR01GM092895 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI LESCHZINER, ANDRES · 2011 to 2021
$3.0M
Protein structure determination from low-resolution experimental dataR01GM123089 · NIGMS · UNIVERSITY OF WASHINGTON · PI DIMAIO, FRANK P · 2017 to 2025
$2.6M
Silicon Mechanics Compute ClusterS10OD020011 · OD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SKINIOTIS, GEORGIOS · 2015 to 2015
$490k
NIGMS NIH HHS R01 GM092895NIGMS NIH HHS R01 GM107214NIGMS NIH HHS R01 GM123089NIH HHS S10 OD020011
6 · The paper itself

Abstract

Access to streamlined computational resources remains a significant bottleneck for new users of cryo-electron microscopy (cryo-EM). To address this, we have developed tools that will submit cryo-EM analysis routines and atomic model building jobs directly to Amazon Web Services (AWS) from a local computer or laptop. These new software tools ("cryoem-cloud-tools") have incorporated optimal data movement, security, and cost-saving strategies, giving novice users access to complex cryo-EM data processing pipelines. Integrating these tools into the RELION processing pipeline and graphical user interface we determined a 2.2 Å structure of ß-galactosidase in ∼55 h on AWS. We implemented a similar strategy to submit Rosetta atomic model building and refinement to AWS. These software tools dramatically reduce the barrier for entry of new users to cloud computing for cryo-EM and are freely available at cryoem-tools.cloud.

Indexed as

Softwarebeta-GalactosidaseComputational BiologyCryoelectron MicroscopyImage Processing, Computer-Assistedbeta-GalactosidaseAmazon Web ServicesCloud computingCryo-electron microscopy

Identifiers

PMID29864529
PMCPMC6091888
OpenAlexW2805436113

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.