ArticlePloS one2018
Pleomorphic linkers as ubiquitous structural organizers of vesicles in axons.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Spatial confinement shapes organelle architecture and remodeling in axons.bioRxiv : the preprint server for biology · 2026Article
- Pathological TDP-43 filaments accumulate at synapses and cause synaptic dysfunction.bioRxiv : the preprint server for biology · 2026Article
- Visualizing Postsynaptic Density in Excitatory Synapses with Electron Tomography.Advances in neurobiology · 2026Review
- Cryo-correlative light and electron tomography of dopaminergic axonal varicosities reveals non-synaptic modulation of cortico-striatal synapses.Nature communications · 2025Article
- Cryo-ET suggests tubulin chaperones form a subset of microtubule lumenal particles with a role in maintaining neuronal microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Handling Difficult Cryo-ET Samples: A Study with Primary Neurons from Drosophila melanogaster.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2023Article
- An Improved Method for Growing Primary Neurons on Electron Microscopy Grids Co-Cultured with Astrocytes.International journal of molecular sciences · 2023Article
- Munc13- and SNAP25-dependent molecular bridges play a key role in synaptic vesicle priming.Science advances · 2023Article
- Lumenal components of cytoplasmic microtubules.Biochemical Society transactions · 2022Review
- Rapid isolation and cryo-EM characterization of synaptic vesicles from mammalian brain.FEBS open bio · 2022Article
- A cryo-ET survey of microtubules and intracellular compartments in mammalian axons.The Journal of cell biology · 2022Article
- Editorial: Quantifying and controlling the nano-architecture of neuronal synapses.Frontiers in synaptic neuroscience · 2022Article
- Neurons as a model system for cryo-electron tomography.Journal of structural biology: X · 2022Article
- Digging Deeper: Advancements in Visualization of Inhibitory Synapses in Neurodegenerative Disorders.International journal of molecular sciences · 2021Review
- Electron cryo-tomography reveals the subcellular architecture of growing axons in human brain organoids.eLife · 2021Article
- Trans-synaptic assemblies link synaptic vesicles and neuroreceptors.Science advances · 2021Article
- Hypothesis Relating the Structure, Biochemistry and Function of Active Zone Material Macromolecules at a Neuromuscular Junction.Frontiers in synaptic neuroscience · 2021Article
- ELKS1 Captures Rab6-Marked Vesicular Cargo in Presynaptic Nerve Terminals.Cell reports · 2020Article
- Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions.Frontiers in cellular neuroscience · 2020Article
- Ultrastructure of the axonal periodic scaffold reveals a braid-like organization of actin rings.Nature communications · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many cellular processes depend on a precise structural organization of molecular components. Here, we established that neurons grown in culture provide a suitable system for in situ structural investigations of cellular structures by cryo-electron tomography, a method that allows high resolution, three-dimensional imaging of fully hydrated, vitrified cellular samples. A higher level of detail of cellular components present in our images allowed us to quantitatively characterize presynaptic and cytoskeletal organization, as well as structures involved in axonal transport and endocytosis. In this way we provide a structural framework into which information from other methods need to fit. Importantly, we show that short pleomorphic linkers (tethers and connectors) extensively interconnect different types of spherical vesicles and other lipid membranes in neurons imaged in a close-to-native state. These linkers likely serve to organize and precisely position vesicles involved in endocytosis, axonal transport and synaptic release. Hence, structural interactions via short linkers may serve as ubiquitous vesicle organizers in neuronal cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.