ArticleJournal of visualized experiments : JoVE2018
Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Matrix stress relaxation drives glioblastoma cell response in viscoelastic biomaterials.Science advances · 2026Article
- Stress relaxation timescale and hydrogel network connectivity regulate neural progenitor cell stemness and differentiation.Journal of materials chemistry. B · 2026Article
- Tuning viscoelasticity of dynamic covalent hydrogels for human tissue modeling.bioRxiv : the preprint server for biology · 2025Article
- Submucosal Hydrogel for Spring-Mediated Intestinal Lengthening.Journal of biomedical materials research. Part A · 2025Article
- Viscoelastic N‑cadherin-like interactions maintain neural progenitor cell stemness within 3D matrices.Nature communications · 2025Article
- Engineered Hydrogels for Organoid Models of Human Nonalcoholic Fatty Liver Disease.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Bioorthogonal Engineering of Cellular Microenvironments Using Isonitrile Ligations.Advanced functional materials · 2025Article
- Engineered matrices reveal stiffness-mediated chemoresistance in patient-derived pancreatic cancer organoids.Nature materials · 2024Article
- Cell Microencapsulation Within Engineered Hyaluronan Elastin-Like Protein (HELP) Hydrogels.Current protocols · 2023Article
- Tunable hydrogel viscoelasticity modulates human neural maturation.Science advances · 2023Article
- Design Parameters for Injectable Biopolymeric Hydrogels with Dynamic Covalent Chemistry Crosslinks.Advanced healthcare materials · 2023Article
- Elastin-like protein hydrogels with controllable stress relaxation rate and stiffness modulate endothelial cell function.Journal of biomedical materials research. Part A · 2023Article
- 3D bioprinting of dynamic hydrogel bioinks enabled by small molecule modulators.Science advances · 2023Article
- Nanocomposite Hydrogels as Functional Extracellular Matrices.Gels (Basel, Switzerland) · 2023Review
- Tuning Polymer Hydrophilicity to Regulate Gel Mechanics and Encapsulated Cell Morphology.Advanced healthcare materials · 2022Article
- Hyaluronan and elastin-like protein (HELP) gels significantly improve microsphere retention in the myocardium.Biomaterials science · 2022Article
- Cerebral Embolism as a Result of Facial Filler Injections: A Literature Review.Aesthetic surgery journal · 2022Review
- Elastin-like Proteins to Support Peripheral Nerve Regeneration in Guidance Conduits.ACS biomaterials science & engineering · 2021Article
- Engineered Matrices Enable the Culture of Human Patient-Derived Intestinal Organoids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Neural Progenitor Cells Alter Chromatin Organization and Neurotrophin Expression in Response to 3D Matrix Degradability.Advanced healthcare materials · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Two-dimensional (2D) tissue culture techniques have been essential for our understanding of fundamental cell biology. However, traditional 2D tissue culture systems lack a three-dimensional (3D) matrix, resulting in a significant disconnect between results collected in vitro and in vivo. To address this limitation, researchers have engineered 3D hydrogel tissue culture platforms that can mimic the biochemical and biophysical properties of the in vivo cell microenvironment. This research has motivated the need to develop material platforms that support 3D cell encapsulation and downstream biochemical assays. Recombinant protein engineering offers a unique toolset for 3D hydrogel material design and development by allowing for the specific control of protein sequence and therefore, by extension, the potential mechanical and biochemical properties of the resultant matrix. Here, we present a protocol for the expression of recombinantly-derived elastin-like protein (ELP), which can be used to form hydrogels with independently tunable mechanical properties and cell-adhesive ligand concentration. We further present a methodology for cell encapsulation within ELP hydrogels and subsequent immunofluorescent staining of embedded cells for downstream analysis and quantification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.