A simple microdevice for single cell capture, array, release, and fast staining using oscillatory method.

Microchips that perform single cell capture, array, and identification have become powerful tools for single cell studies, which can reveal precise underlying mechanisms among bulk cell populations. However, current single cell capture and on-chip immunostaining methods consume more time and reagent than desired. To optimize this technology, we designed a novel trap structure for single cell capture, array, and release, and meanwhile an oscillatory method was used to perform rapid on-chip cell immunostaining. The trap structure array used equal distribution of lateral flow to achieve single cell array in high velocity flows and decrease the risk of clogging. A length of glass capillary with a sealed bubble was inserted into the outlet so that it could act in a manner analogous to that of a capacitor in an RC circuit. By applying one periodic air pressure to the inlet, oscillation motion was generated, which significantly enhanced the on-chip reaction efficiency. In addition, the oscillation performance could be easily regulated by changing the length of the capillary. The trapped cells could maintain their positions during oscillation; hence, they were able to be tracked in real time. Through our trap microchip, 12