ArticleProceedings of the National Academy of Sciences of the United States of America1985
Isolation and characterization of an olfactory receptor protein for odorant pyrazines.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 174 citations in OpenAlex.
- Article
- An odorant-binding protein in the elephant's trunk is finely tuned to sex pheromone (Z)-7-dodecenyl acetate.Scientific reports · 2022Article
- Lipocalins in Arthropod Chemical Communication.Genome biology and evolution · 2021Article
- Effects of time and temperature during melanging on the volatile profile of dark chocolate.Scientific reports · 2020Article
- Significance and Transformation of 3-Alkyl-2-Methoxypyrazines Through Grapes to Wine: Olfactory Properties, Metabolism, Biochemical Regulation, and the HP-MP Cycle.Molecules (Basel, Switzerland) · 2019Review
- Sense of Smell: Structural, Functional, Mechanistic Advancements and Challenges in Human Olfactory Research.Current neuropharmacology · 2019Article
- Buffalo nasal odorant-binding protein (bunOBP) and its structural evaluation with putative pheromones.Scientific reports · 2018Article
- Reverse chemical ecology: Olfactory proteins from the giant panda and their interactions with putative pheromones and bamboo volatiles.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Structure and stability of recombinant bovine odorant-binding protein: II. Unfolding of the monomeric forms.PeerJ · 2016Article
- An odorant-binding protein is abundantly expressed in the nose and in the seminal fluid of the rabbit.PloS one · 2014Article
- Soluble proteins of chemical communication: an overview across arthropods.Frontiers in physiology · 2014Review
- Biomimetic chemical sensors using nanoelectronic readout of olfactory receptor proteins.ACS nano · 2011Article
- Mind molecules.The Journal of biological chemistry · 2011Article
- First Contact to Odors: Our Current Knowledge about Odorant Receptor.Sensors (Basel, Switzerland) · 2008Review
- Olfactory regulation of mosquito-host interactions.Insect biochemistry and molecular biology · 2004Review
- Odor space and olfactory processing: collective algorithms and neural implementation.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- The lipocalin protein family: structure and function.The Biochemical journal · 1996Review
- Membrane receptor for odour-binding proteins.The Biochemical journal · 1996Article
- Molecular genetics of mammalian olfaction.Behavior genetics · 1996Review
- cDNA sequence and expression pattern of the putative pheromone carrier aphrodisin.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
The highly potent bell pepper odorant 2-isobutyl-3-[3H]methoxypyrazine [( 3H]IBMP) binds specifically and saturably to bovine and rat nasal epithelium. Specific binding is not detected in 11 other tissues assayed, and in the rat binding is 9 times higher in olfactory than in respiratory epithelium. We have purified to apparent homogeneity a soluble pyrazine odorant binding protein that constitutes approximately equal to 1% of the total soluble protein in bovine nasal epithelium. Polyacrylamide gel electrophoresis shows a single band of 19,000 Da and gel filtration data suggest that the native protein is a dimer of 38,000 Da. Binding of [3H]IBMP to the purified protein reveals two binding sites (Kd = 10 X 10(-9) M, Bmax = 135 pmol per mg of protein; Kd = 3 X 10(-6) M, Bmax = 25 nmol per mg of protein). The binding affinities of a homologous series of pyrazine odorants correlate with the human odor detection thresholds of these compounds. This correlation, together with the regional distribution of the protein, suggests that the protein is a physiologically relevant olfactory receptor.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.