ArticleLasers in medical science2018
Near infrared laser irradiation induces NETosis via oxidative stress and autophagy.
Article in Lasers in medical science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
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- Neutrophil extracellular traps mediated by platelet microvesicles promote thrombosis and brain injury in acute ischemic stroke.Cell communication and signaling : CCS · 2024Article
- Laser Biostimulation Induces Wound Healing-Promoter β2-Defensin Expression in Human Keratinocytes via Oxidative Stress.Antioxidants (Basel, Switzerland) · 2023Article
- Radiation from UV-A to Red Light Induces ROS-Dependent Release of Neutrophil Extracellular Traps.International journal of molecular sciences · 2023Article
- Photobiomodulation induces microvesicle release in human keratinocytes: PI3 kinase-dependent pathway role.Lasers in medical science · 2022Article
- Protective Effect of Photobiomodulation against Hydrogen Peroxide-Induced Oxidative Damage by Promoting Autophagy through Inhibition of PI3K/AKT/mTOR Pathway in MC3T3-E1 Cells.Oxidative medicine and cellular longevity · 2022Article
- Spontaneous formation of neutrophil extracellular traps is associated with autophagy.Scientific reports · 2021Article
- Blue and Long-Wave Ultraviolet Light InduceFrontiers in immunology · 2019Article
- Bovine neutrophils form extracellular traps in response to the gastrointestinal parasite Ostertagia ostertagi.Scientific reports · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NETosis is a novel immune defense strategy in which neutrophil activation results in the formation of extracellular DNA/protein network which is able to kill microbial populations. NETosis can be induced in vitro by lipopolysaccharide (LPS) or phorbol myristate acetate (PMA). Due to the importance of NETosis in different physiological and pathological processes, photobiostimulation effect on this neutrophil activation mechanism has been investigated. Human granulocytes, isolated from venous blood of healthy donors, were stimulated with a diode laser emitting at 980 nm with an energy intensity ranging from 0 to 75 joules. After 3 h of laser stimulation, granulocytes were fixed and colored with crystal violet in order to assess the NETosis morphology while extracellular DNA produced has been quantified using Sytox Green fluorescent dye. To evaluate ROS production and autophagy role in photobiostimulation-induced NETosis, granulocytes were pre-treated with ROS scavengers (vitamin C, sodium pyruvate, L-NAME, sodium azide), and an autophagy inhibitor (wortmannin). Laser stimulation induced an energy-dependent neutrophil extracellular trap (NET) production in human granulocytes starting from 50-J laser intensity. ROS scavengers and the autophagy inhibitor were able to abrogate both morphological features of NETosis and extracellular DNA production without modifying the basal level of NETosis. Photobiostimulation induced an increase in NET production due to an increase in ROS levels and autophagy activation.
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